Di Fabrizio, Enzo M.
KAUST DepartmentPhysical Sciences and Engineering (PSE) Division
Imaging and Characterization Core Lab
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AbstractIn this work, micro-fabrication, super-hydrophobic properties and a physiologically compatible preparation step are combined and tailored to obtain background free biological samples to be investigated by Transmission Electron Microscopy (TEM) diffraction technique. The validation was performed evaluating a well-known parameter such as the DNA interbases value. The diffraction spacing measured is in good agreement with those obtained by HRTEM direct metrology and by traditional X-Ray diffraction. This approach addresses single molecule studies in a simplified and reproducible straightforward way with respect to more conventional and widely used techniques. In addition, it overcomes the need of long and elaborated samples preparations: the sample is in its physiological environment and the HRTEM data acquisition occurs without any background interference, coating, staining or additional manipulation. The congruence in the results reported in this paper makes the application of this approach extremely promising towards those molecules for which crystallization remains a hurdle, such as cell membrane proteins and fibrillar proteins.
CitationMarini M, Allione M, Lopatin S, Moretti M, Giugni A, et al. (2017) Suspended DNA structural characterization by TEM diffraction. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2017.11.020.
SponsorsThe authors acknowledge financial support from the KAUST start-up funding and from Italian Ministry of Health under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.