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    Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation

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    1-s2.0-S0734975017301507-main.pdf
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    PDF
    Description:
    Accepted Manuscript
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    Type
    Article
    Authors
    Mahas, Ahmed
    Neal Stewart, C.
    Mahfouz, Magdy M. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Laboratory for Genome Engineering
    Date
    2017-11-29
    Online Publication Date
    2017-11-29
    Print Publication Date
    2018-01
    Permanent link to this record
    http://hdl.handle.net/10754/626280
    
    Metadata
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    Abstract
    Genome editing has enabled broad advances and novel approaches in studies of gene function and structure; now, emerging methods aim to precisely engineer post-transcriptional processes. Developing precise, efficient molecular tools to alter the transcriptome holds great promise for biotechnology and synthetic biology applications. Different approaches have been employed for targeted degradation of RNA species in eukaryotes, but they lack programmability and versatility, thereby limiting their utility for diverse applications. The CRISPR/Cas9 system has been harnessed for genome editing in many eukaryotic species and, using a catalytically inactive Cas9 variant, the CRISPR/dCas9 system has been repurposed for transcriptional regulation. Recent studies have used other CRISPR/Cas systems for targeted RNA degradation and RNA-based manipulations. For example, Cas13a, a Type VI-A endonuclease, has been identified as an RNA-guided RNA ribonuclease and used for manipulation of RNA. Here, we discuss different modalities for targeted RNA interference with an emphasis on the potential applications of CRISPR/Cas systems as programmable transcriptional regulators for broad uses, including functional biology, biotechnology, and synthetic biology applications.
    Citation
    Mahas A, Neal Stewart C, Mahfouz MM (2017) Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation. Biotechnology Advances. Available: http://dx.doi.org/10.1016/j.biotechadv.2017.11.008.
    Sponsors
    This study was supported by King Abdullah University of Science and Technology (KAUST).
    Publisher
    Elsevier BV
    Journal
    Biotechnology Advances
    DOI
    10.1016/j.biotechadv.2017.11.008
    PubMed ID
    29197619
    Additional Links
    http://www.sciencedirect.com/science/article/pii/S0734975017301507
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.biotechadv.2017.11.008
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program

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