Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation
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ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Laboratory for Genome Engineering
Date
2017-11-29Online Publication Date
2017-11-29Print Publication Date
2018-01Permanent link to this record
http://hdl.handle.net/10754/626280
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Genome editing has enabled broad advances and novel approaches in studies of gene function and structure; now, emerging methods aim to precisely engineer post-transcriptional processes. Developing precise, efficient molecular tools to alter the transcriptome holds great promise for biotechnology and synthetic biology applications. Different approaches have been employed for targeted degradation of RNA species in eukaryotes, but they lack programmability and versatility, thereby limiting their utility for diverse applications. The CRISPR/Cas9 system has been harnessed for genome editing in many eukaryotic species and, using a catalytically inactive Cas9 variant, the CRISPR/dCas9 system has been repurposed for transcriptional regulation. Recent studies have used other CRISPR/Cas systems for targeted RNA degradation and RNA-based manipulations. For example, Cas13a, a Type VI-A endonuclease, has been identified as an RNA-guided RNA ribonuclease and used for manipulation of RNA. Here, we discuss different modalities for targeted RNA interference with an emphasis on the potential applications of CRISPR/Cas systems as programmable transcriptional regulators for broad uses, including functional biology, biotechnology, and synthetic biology applications.Citation
Mahas A, Neal Stewart C, Mahfouz MM (2017) Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation. Biotechnology Advances. Available: http://dx.doi.org/10.1016/j.biotechadv.2017.11.008.Sponsors
This study was supported by King Abdullah University of Science and Technology (KAUST).Publisher
Elsevier BVJournal
Biotechnology AdvancesPubMed ID
29197619Additional Links
http://www.sciencedirect.com/science/article/pii/S0734975017301507ae974a485f413a2113503eed53cd6c53
10.1016/j.biotechadv.2017.11.008
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