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dc.contributor.authorSchrittwieser, Stefan
dc.contributor.authorPelaz, Beatriz
dc.contributor.authorParak, Wolfgang J.
dc.contributor.authorLentijo Mozo, Sergio
dc.contributor.authorSoulantica, Katerina
dc.contributor.authorDieckhoff, Jan
dc.contributor.authorLudwig, Frank
dc.contributor.authorSchotter, Joerg
dc.date.accessioned2017-10-31T08:20:20Z
dc.date.available2017-10-31T08:20:20Z
dc.date.issued2017-06-30
dc.identifier.citationSchrittwieser S, Pelaz B, Parak WJ, Lentijo-Mozo S, Soulantica K, et al. (2017) Direct protein quantification in complex sample solutions by surface-engineered nanorod probes. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-04970-5.
dc.identifier.issn2045-2322
dc.identifier.pmid28684848
dc.identifier.doi10.1038/s41598-017-04970-5
dc.identifier.urihttp://hdl.handle.net/10754/626063
dc.description.abstractDetecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence.
dc.description.sponsorshipWe thank Frauke Alves and Julia Bode from the Max-Planck-Institute of Experimental Medicine in Göttingen for the supply with the Herceptin therapeutic drug. We also want to thank David Fernandez and Amagoia Ametzazurra from Progenika Biopharma for providing the serum. This research was supported by the European Commission FP7 NAMDIATREAM project (EU NMP4-LA-2010–246479), and the German Research Foundation (DFG grant PA 794/25-1).
dc.publisherSpringer Nature
dc.relation.urlhttps://www.nature.com/articles/s41598-017-04970-5
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleDirect protein quantification in complex sample solutions by surface-engineered nanorod probes
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMolecular Diagnostics, AIT Austrian Institute of Technology, Vienna, , Austria
dc.contributor.institutionCentro Singular de Investigacion en Quimica Biologica y Materiales Moleculares (CiQUS), Departamento de Física de Partículas, Universidade de Santiago de Compostela, Santiago de Compostela, , Spain
dc.contributor.institutionFachbereich Physik, Philipps-Universität Marburg, Marburg, , Germany
dc.contributor.institutionCIC Biomagune, San Sebastian, , Spain
dc.contributor.institutionLaboratoire de Physique et Chimie des Nano-objets (LPCNO), Université de Toulouse, INSA, UPS, CNRS, Toulouse, , , , , France
dc.contributor.institutionDiagnostic and Interventional Radiology Department and Clinic, University Medical Center Hamburg-Eppendorf, Hamburg, , Germany
dc.contributor.institutionInstitute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Braunschweig, , Germany
kaust.personLentijo Mozo, Sergio
refterms.dateFOA2018-06-13T10:49:23Z


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.