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dc.contributor.authorAli, Zahir
dc.contributor.authorEid, Ayman
dc.contributor.authorAli, Shawkat
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2017-10-30T08:39:52Z
dc.date.available2017-10-30T08:39:52Z
dc.date.issued2017-10-17
dc.identifier.citationAli Z, Eid A, Ali S, Mahfouz MM (2017) Pea early-browning virus -mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis. Virus Research. Available: http://dx.doi.org/10.1016/j.virusres.2017.10.009.
dc.identifier.issn0168-1702
dc.identifier.pmid29051052
dc.identifier.doi10.1016/j.virusres.2017.10.009
dc.identifier.urihttp://hdl.handle.net/10754/626046
dc.description.abstractThe clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system has enabled efficient genome engineering in diverse plant species. However, delivery of genome engineering reagents, such as the single guide RNA (sgRNA), into plant cells remains challenging. Here, we report the engineering of Tobacco rattle virus (TRV) and Pea early browning virus (PEBV) to deliver one or multiple sgRNAs into Nicotiana benthamiana and Arabidopsis thaliana (Col-0) plants that overexpress a nuclear localization signal containing Cas9. Our data showed that TRV and PEBV can deliver sgRNAs into inoculated and systemic leaves, and this resulted in mutagenesis of the targeted genomic loci. Moreover, in N. benthamiana, PEBV-based sgRNA delivery resulted in more targeted mutations than TRV-based delivery. Our data indicate that TRV and PEBV can facilitate plant genome engineering and can be used to produce targeted mutations for functional analysis and other biotechnological applications across diverse plant species.Key message: Delivery of genome engineering reagents into plant cells is challenging and inefficient and this limit the applications of this technology in many plant species. RNA viruses such as TRV and PEBV provide an efficient tool to systemically deliver sgRNAs for targeted genome modification.
dc.description.sponsorshipWe would like to thank member of the laboratory for genome engineering for continuous discussions. We would like to thank Professor Elisabeth Johansen, Danish Institute for food and veterinary research, for providing PEBV-containing binary constructs. This study is supported by King Abdullah University of Science and Technology (KAUST).
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0168170217305543
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Virus Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Virus Research, [, , (2017-10-17)] DOI: 10.1016/j.virusres.2017.10.009 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCRISPR/Cas9
dc.subjectVirus-mediated Genome Engineering
dc.subjectVirus-mediated Genome Editing
dc.subjectTargeted Mutagenesis
dc.subjectTobacco rattle virus
dc.subjectPea early browning virus
dc.subjectArabidopsis
dc.subjectNicotiana benthamiana
dc.titlePea early-browning virus -mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science Program
dc.contributor.departmentLaboratory for Genome Engineering, Division of Environmental and Biological Sciences and Engineering, 4700 King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia
dc.identifier.journalVirus Research
dc.eprint.versionPost-print
kaust.personAli, Zahir
kaust.personEid, Ayman
kaust.personAli, Shawkat
kaust.personMahfouz, Magdy M.


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