Removal of antibiotic-resistant bacteria and antibiotic resistance genes affected by varying degrees of fouling on anaerobic microfiltration membranes
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant NumberFCC/1/1971-06-01
MetadataShow full item record
AbstractAn anaerobic membrane bioreactor was retrofitted with polyvinylidene fluoride (PVDF) microfiltration membrane units, each of which was fouled to a different extent. The membranes with different degrees of fouling were evaluated for their efficiencies in removing three antibiotic-resistant bacteria (ARB), namely, blaNDM-1-positive Escherichia coli PI-7, blaCTX-M-15-positive Klebsiella pneumoniae L7, and blaOXA-48-positive E. coli UPEC-RIY-4, as well as their associated plasmid-borne antibiotic resistance genes (ARGs). The results showed that the log removal values (LRVs) of ARGs correlated positively with the extent of membrane fouling and ranged from 1.9 to 3.9. New membranes with a minimal foulant layer could remove more than 5 log units of ARB. However, as the membranes progressed to subcritical fouling, the LRVs of ARB decreased at increasing operating transmembrane pressures (TMPs). The LRV recovered back to 5 when the membrane was critically fouled, and the achieved LRV remained stable at different operating TMPs. Furthermore, characterization of the surface attributed the removal of both the ARB and ARGs to adsorption, which was facilitated by an increasing hydrophobicity and a decreasing surface ζ potential as the membranes fouled. Our results indicate that both the TMP and the foulant layer synergistically affected ARB removal, but the foulant layer was the main factor that contributed to ARG removal.
CitationCheng H, Hong P-Y (2017) Removal of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes Affected by Varying Degrees of Fouling on Anaerobic Microfiltration Membranes. Environmental Science & Technology. Available: http://dx.doi.org/10.1021/acs.est.7b03798.
SponsorsThe authors thank Mr. Qingtian Guan for providing technical assistance with plasmid assemblies. The authors thank Dr. Tiannyu Wang for creating the GFP-positive E. coli PI-7 strain. This study is supported by KAUST Center Competitive Funding, grant no. FCC/1/1971-06-01, awarded to P.-Y.H.
PublisherAmerican Chemical Society (ACS)
- Effects of electron acceptors on removal of antibiotic resistant Escherichia coli, resistance genes and class 1 integrons under anaerobic conditions.
- Authors: Yuan H, Miller JH, Abu-Reesh IM, Pruden A, He Z
- Issue date: 2016 Nov 1
- H<sub>2</sub>O<sub>2</sub> and/or TiO<sub>2</sub> photocatalysis under UV irradiation for the removal of antibiotic resistant bacteria and their antibiotic resistance genes.
- Authors: Guo C, Wang K, Hou S, Wan L, Lv J, Zhang Y, Qu X, Chen S, Xu J
- Issue date: 2017 Feb 5
- Effect of microfiltration concentration factor on serum protein removal from skim milk using spiral-wound polymeric membranes.
- Authors: Beckman SL, Barbano DM
- Issue date: 2013 Oct
- Removal of microbial indicators from municipal wastewater by a membrane bioreactor (MBR).
- Authors: Marti E, Monclús H, Jofre J, Rodriguez-Roda I, Comas J, Balcázar JL
- Issue date: 2011 Apr
- Removal of coliphages in secondary effluent by microfiltration-mechanisms of removal and impact of operating parameters.
- Authors: Farahbakhsh K, Smith DW
- Issue date: 2004 Feb