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dc.contributor.authorChen, Jit Ern
dc.contributor.authorBarbrook, Adrian C.
dc.contributor.authorCui, Guoxin
dc.contributor.authorHowe, Christopher J.
dc.contributor.authorAranda, Manuel
dc.date.accessioned2019-02-24T08:27:04Z
dc.date.available2017-10-17T08:48:35Z
dc.date.available2019-02-24T08:27:04Z
dc.date.issued2019-02-19
dc.identifier.citationChen JE, Barbrook AC, Cui G, Howe CJ, Aranda M (2019) The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods. PLOS ONE 14: e0211936. Available: http://dx.doi.org/10.1371/journal.pone.0211936.
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0211936
dc.identifier.doi10.1101/140616
dc.identifier.urihttp://hdl.handle.net/10754/625875
dc.description.abstractModern transformation and genome editing techniques have shown great success across a broad variety of organisms. However, no study of successfully applied genome editing has been reported in a dinoflagellate despite the first genetic transformation of Symbiodinium being published about 20 years ago. Using an array of different available transformation techniques, we attempted to transform Symbiodinium microadriaticum (CCMP2467), a dinoflagellate symbiont of reef-building corals, with the view to performing subsequent CRISPR-Cas9 mediated genome editing. Plasmid vectors designed for nuclear transformation containing the chloramphenicol resistance gene under the control of the CaMV p35S promoter as well as several putative endogenous promoters were used to test a variety of transformation techniques including biolistics, electroporation and agitation with silicon carbide whiskers. Chloroplast-targeted transformation was attempted using an engineered Symbiodinium chloroplast minicircle encoding a modified PsbA protein expected to confer atrazine resistance. We report that we have been unable to confer chloramphenicol or atrazine resistance on Symbiodinium microadriaticum strain CCMP2467.
dc.description.sponsorshipFunded by King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR). https://www.kaust.edu.sa/en/research/sponsored-research URF/1/1705-01. MA. King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research. (OSR). https://www.kaust.edu.sa/en/research/sponsored-research OCRF-2014-CRG3-2216. MA, CJH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211936
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentMarine Science Program
dc.identifier.journalPLOS ONE
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionJeffrey Sachs Center on Sustainable Development, Sunway University, Bandar Sunway, Malaysia
dc.contributor.institutionDepartment of Biological Sciences, Sunway University, Bandar Sunway, Malaysia
dc.contributor.institutionDepartment of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
kaust.personChen, Jit Ern
kaust.personCui, Guoxin
kaust.personAranda, Manuel
kaust.grant.numberURF/1/1705-01
kaust.grant.numberOCRF-2014-CRG3-2216
refterms.dateFOA2018-06-13T21:34:26Z


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