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    In-line balanced detection stimulated Raman scattering microscopy

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    Type
    Article
    Authors
    Crisafi, Francesco cc
    Kumar, Vikas
    Scopigno, Tullio
    Marangoni, Marco
    Cerullo, Giulio
    Polli, Dario cc
    Date
    2017-09-06
    Online Publication Date
    2017-09-06
    Print Publication Date
    2017-12
    Permanent link to this record
    http://hdl.handle.net/10754/625800
    
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    Abstract
    We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.
    Citation
    Crisafi F, Kumar V, Scopigno T, Marangoni M, Cerullo G, et al. (2017) In-line balanced detection stimulated Raman scattering microscopy. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-09839-1.
    Sponsors
    This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines.
    Publisher
    Springer Nature
    Journal
    Scientific Reports
    DOI
    10.1038/s41598-017-09839-1
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-017-09839-1
    Scopus Count
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