Arabidopsis ATRX Modulates H3.3 Occupancy and Fine-Tunes Gene Expression
Le Goff, Samuel
Probst, Aline V.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Chromatin and development Research Group
Plant Science Program
Online Publication Date2017-07-06
Print Publication Date2017-07
Permanent link to this recordhttp://hdl.handle.net/10754/625666
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AbstractHistones are essential components of the nucleosome, the major chromatin subunit that structures linear DNA molecules and regulates access of other proteins to DNA. Specific histone chaperone complexes control the correct deposition of canonical histones and their variants to modulate nucleosome structure and stability. In this study, we characterize the Arabidopsis Alpha Thalassemia-mental Retardation X-linked (ATRX) ortholog and show that ATRX is involved in histone H3 deposition. Arabidopsis ATRX mutant alleles are viable, but show developmental defects and reduced fertility. Their combination with mutants of the histone H3.3 chaperone HIRA (Histone Regulator A) results in impaired plant survival, suggesting that HIRA and ATRX function in complementary histone deposition pathways. Indeed, ATRX loss of function alters cellular histone H3.3 pools and in consequence modulates the H3.1/H3.3 balance in the cell. H3.3 levels are affected especially at genes characterized by elevated H3.3 occupancy, including the 45S ribosomal DNA (45S rDNA) loci, where loss of ATRX results in altered expression of specific 45S rDNA sequence variants. At the genome-wide scale, our data indicate that ATRX modifies gene expression concomitantly to H3.3 deposition at a set of genes characterized both by elevated H3.3 occupancy and high expression. Altogether, our results show that ATRX is involved in H3.3 deposition and emphasize the role of histone chaperones in adjusting genome expression.
CitationDuc C, Benoit M, Détourné G, Simon L, Poulet A, et al. (2017) Arabidopsis ATRX Modulates H3.3 Occupancy and Fine-Tunes Gene Expression. The Plant Cell 29: 1773–1793. Available: http://dx.doi.org/10.1105/tpc.16.00877.
SponsorsWe thank S. Amiard for seeds and help with the EdU detection, HU and gamma irradiation experiments, P. Pouchin and N. Goué for help with bioinformatics analyses, A. Lhomme for technical assistance, E. Vanrobays for the NLS-GFP construct, O. Mittelsten Scheid for critical reading of the manuscript and F. Pontvianne for valuable advice with 45S rDNA variant analysis and for critical reading of the manuscript. The RNA Sequencing was performed by the IGBMC Microarray and Sequencing platform, a member of the ‘France Génomique’ consortium (ANR-10-INBS-0009). This work was supported by doctoral stipends from the Region Auvergne, ANR grant ‘Dynam’Het’ ANR-11 JSV2 009 01 and ‘SINODYN’ ANR-12 ISV6 0001. This work was further supported by the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale and Université Clermont Auvergne.
JournalThe Plant Cell
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