Arabidopsis ATRX Modulates H3.3 Occupancy and Fine-Tunes Gene Expression
Type
ArticleAuthors
Duc, Céline
Benoit, Matthias

Détourné, Gwénaëlle

Simon, Lauriane

Poulet, Axel

Jung, Matthieu

Veluchamy, Alaguraj

Latrasse, David

Le Goff, Samuel

Cotterell, Sylviane

Tatout, Christophe

Benhamed, Moussa

Probst, Aline V.

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionDesert Agriculture Initiative
Plant Science
Plant Science Program
Date
2017-07-06Online Publication Date
2017-07-06Print Publication Date
2017-07Permanent link to this record
http://hdl.handle.net/10754/625666
Metadata
Show full item recordAbstract
Histones are essential components of the nucleosome, the major chromatin subunit that structures linear DNA molecules and regulates access of other proteins to DNA. Specific histone chaperone complexes control the correct deposition of canonical histones and their variants to modulate nucleosome structure and stability. In this study, we characterize the Arabidopsis Alpha Thalassemia-mental Retardation X-linked (ATRX) ortholog and show that ATRX is involved in histone H3 deposition. Arabidopsis ATRX mutant alleles are viable, but show developmental defects and reduced fertility. Their combination with mutants of the histone H3.3 chaperone HIRA (Histone Regulator A) results in impaired plant survival, suggesting that HIRA and ATRX function in complementary histone deposition pathways. Indeed, ATRX loss of function alters cellular histone H3.3 pools and in consequence modulates the H3.1/H3.3 balance in the cell. H3.3 levels are affected especially at genes characterized by elevated H3.3 occupancy, including the 45S ribosomal DNA (45S rDNA) loci, where loss of ATRX results in altered expression of specific 45S rDNA sequence variants. At the genome-wide scale, our data indicate that ATRX modifies gene expression concomitantly to H3.3 deposition at a set of genes characterized both by elevated H3.3 occupancy and high expression. Altogether, our results show that ATRX is involved in H3.3 deposition and emphasize the role of histone chaperones in adjusting genome expression.Citation
Duc C, Benoit M, Détourné G, Simon L, Poulet A, et al. (2017) Arabidopsis ATRX Modulates H3.3 Occupancy and Fine-Tunes Gene Expression. The Plant Cell 29: 1773–1793. Available: http://dx.doi.org/10.1105/tpc.16.00877.Sponsors
We thank S. Amiard for seeds and help with the EdU detection, HU and gamma irradiation experiments, P. Pouchin and N. Goué for help with bioinformatics analyses, A. Lhomme for technical assistance, E. Vanrobays for the NLS-GFP construct, O. Mittelsten Scheid for critical reading of the manuscript and F. Pontvianne for valuable advice with 45S rDNA variant analysis and for critical reading of the manuscript. The RNA Sequencing was performed by the IGBMC Microarray and Sequencing platform, a member of the ‘France Génomique’ consortium (ANR-10-INBS-0009). This work was supported by doctoral stipends from the Region Auvergne, ANR grant ‘Dynam’Het’ ANR-11 JSV2 009 01 and ‘SINODYN’ ANR-12 ISV6 0001. This work was further supported by the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale and Université Clermont Auvergne.Journal
The Plant CellPubMed ID
28684426Additional Links
http://www.plantcell.org/content/early/2017/07/06/tpc.16.00877ae974a485f413a2113503eed53cd6c53
10.1105/tpc.16.00877
Scopus Count
Related articles
- The histone chaperone complex HIR maintains nucleosome occupancy and counterbalances impaired histone deposition in CAF-1 complex mutants.
- Authors: Duc C, Benoit M, Le Goff S, Simon L, Poulet A, Cotterell S, Tatout C, Probst AV
- Issue date: 2015 Mar
- LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2.
- Authors: Wang H, Jiang D, Axelsson E, Lorković ZJ, Montgomery S, Holec S, Pieters BJGE, Al Temimi AHK, Mecinović J, Berger F
- Issue date: 2018 Aug 6
- PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX.
- Authors: Delbarre E, Ivanauskiene K, Spirkoski J, Shah A, Vekterud K, Moskaug JØ, Bøe SO, Wong LH, Küntziger T, Collas P
- Issue date: 2017 Jun
- Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes.
- Authors: Pavlištová V, Dvořáčková M, Jež M, Mozgová I, Mokroš P, Fajkus J
- Issue date: 2016 Nov
- AtFKBP53 is a histone chaperone required for repression of ribosomal RNA gene expression in Arabidopsis.
- Authors: Li H, Luan S
- Issue date: 2010 Mar