A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Desert Agriculture Initiative
Office of the VP
Plant Science
Date
2017-09-08Online Publication Date
2017-09-08Print Publication Date
2017-11Permanent link to this record
http://hdl.handle.net/10754/625446
Metadata
Show full item recordAbstract
Long non-coding RNAs (lncRNAs) affect gene expression through a wide range of mechanisms and are considered as important regulators in many essential biological processes. A large number of lncRNA transcripts have been predicted or identified in plants in recent years. However, the biological functions for most of them are still unknown. In this study, we identified an Arabidopsis thaliana lncRNA, Drought induced RNA (DRIR), as a novel positive regulator of plant response to drought and salt stress. DRIR was expressed at a low level under non-stress conditions but can be significantly activated by drought and salt stress as well as by abscisic acid (ABA) treatment. We identified a T-DNA insertion mutant, drirD, which had higher expression of the DRIR gene than the wild type plants. The drirD mutant exhibits increased tolerance to drought and salt stress. Overexpressing DRIR in Arabidopsis also increased tolerance to drought and salt stress of the transgenic plants. The drirD mutant and the overexpressing seedlings are more sensitive to ABA than the wild type in stomata closure and seedling growth. Genome-wide transcriptome analysis demonstrated that the expression of a large number of genes was altered in drirD and the overexpressing plants. These include genes involved in ABA signaling, water transport and other stress-relief processes. Our study reveals a mechanism whereby DRIR regulates plant response to abiotic stress by modulating the expression of a series of genes involved in stress response.Citation
Qin T, Zhao H, Cui P, Albesher N, Xiong L (2017) A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance. Plant Physiology: pp.00574.2017. Available: http://dx.doi.org/10.1104/pp.17.00574.Sponsors
We thank the Arabidopsis Biological Resource Center for providing the T-DNA insertion lines.Journal
Plant PhysiologyPubMed ID
28887353Additional Links
http://www.plantphysiol.org/content/early/2017/09/08/pp.17.00574ae974a485f413a2113503eed53cd6c53
10.1104/pp.17.00574
Scopus Count
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