A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses
Permanent link to this recordhttp://hdl.handle.net/10754/625375
MetadataShow full item record
AbstractThe plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H₂O₂)-dependent proteins, little is known about the ABA- and H₂O₂-dependent microsomal proteome changes. Here, we examined the effect of 50 µM of either H₂O₂ or ABA on the Arabidopsis microsomal proteome using tandem mass spectrometry and identified 86 specifically H₂O₂-dependent, and 52 specifically ABA-dependent proteins that are differentially expressed. We observed differential accumulation of proteins involved in the tricarboxylic acid (TCA) cycle notably in response to H₂O₂. Of these, aconitase 3 responded to both H₂O₂ and ABA. Additionally, over 30 proteins linked to RNA biology responded significantly to both treatments. Gene ontology categories such as 'response to stress' and 'transport' were enriched, suggesting that H₂O₂ or ABA directly and/or indirectly cause complex and partly overlapping cellular responses. Data are available via ProteomeXchange with identifier PXD006513.
CitationAlqurashi M, Thomas L, Gehring C, Marondedze C (2017) A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses. Proteomes 5: 22. Available: http://dx.doi.org/10.3390/proteomes5030022.
SponsorsThe authors would like to thank Bothayna Algashgari for technical assistance. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE  partner repository with the dataset identifier PXD006513.
Except where otherwise noted, this item's license is described as This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
- Quantitative transcriptomic analysis of abscisic acid-induced and reactive oxygen species-dependent expression changes and proteomic profiling in Arabidopsis suspension cells.
- Authors: Böhmer M, Schroeder JI
- Issue date: 2011 Jul
- Conservation between higher plants and the moss Physcomitrella patens in response to the phytohormone abscisic acid: a proteomics analysis.
- Authors: Wang X, Kuang T, He Y
- Issue date: 2010 Aug 27
- Arabidopsis HY1-Modulated Stomatal Movement: An Integrative Hub Is Functionally Associated with ABI4 in Dehydration-Induced ABA Responsiveness.
- Authors: Xie Y, Mao Y, Duan X, Zhou H, Lai D, Zhang Y, Shen W
- Issue date: 2016 Mar
- ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana.
- Authors: Sakamoto H, Matsuda O, Iba K
- Issue date: 2008 Nov
- Quantitative proteomics-based analysis supports a significant role of GTG proteins in regulation of ABA response in Arabidopsis roots.
- Authors: Alvarez S, Roy Choudhury S, Hicks LM, Pandey S
- Issue date: 2013 Mar 1