NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato
AuthorsThirumalaikumar, Venkatesh P.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
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AbstractWater deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species, and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus-induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2 O2 ) levels, and a decrease of the expression of various drought-responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2 O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2, and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress-related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato. This article is protected by copyright. All rights reserved.
CitationThirumalaikumar VP, Devkar V, Mehterov N, Ali S, Ozgur R, et al. (2017) NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato. Plant Biotechnology Journal. Available: http://dx.doi.org/10.1111/pbi.12776.
SponsorsThis work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG) to S.B. (BA 4769/2-1). Financial support was furthermore provided by the University of Potsdam and the MPI of Molecular Plant Physiology.
JournalPlant Biotechnology Journal
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- Issue date: 2017
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- Issue date: 2012 Feb
- <i>Arabidopsis</i> NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato.
- Authors: Shahnejat-Bushehri S, Allu AD, Mehterov N, Thirumalaikumar VP, Alseekh S, Fernie AR, Mueller-Roeber B, Balazadeh S
- Issue date: 2017
- The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato (Solanum lycopersicum).
- Authors: Zhu M, Chen G, Zhang J, Zhang Y, Xie Q, Zhao Z, Pan Y, Hu Z
- Issue date: 2014 Nov
- SlDREB2, a tomato dehydration-responsive element-binding 2 transcription factor, mediates salt stress tolerance in tomato and Arabidopsis.
- Authors: Hichri I, Muhovski Y, Clippe A, Žižková E, Dobrev PI, Motyka V, Lutts S
- Issue date: 2016 Jan