Nitrogen Fixation Aligns with nifH Abundance and Expression in Two Coral Trophic Functional Groups
Voolstra, Christian R.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Red Sea Research Center (RSRC)
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AbstractMicrobial nitrogen fixation (diazotrophy) is a functional trait widely associated with tropical reef-building (scleractinian) corals. While the integral role of nitrogen fixation in coral nutrient dynamics is recognized, its ecological significance across different coral functional groups remains yet to be evaluated. Here we set out to compare molecular and physiological patterns of diazotrophy (i.e., nifH gene abundance and expression as well as nitrogen fixation rates) in two coral families with contrasting trophic strategies: highly heterotrophic, free-living members of the family Fungiidae (Pleuractis granulosa, Ctenactis echinata), and mostly autotrophic coral holobionts with low heterotrophic capacity (Pocilloporidae: Pocillopora verrucosa, Stylophora pistillata). The Fungiidae exhibited low diazotroph abundance (based on nifH gene copy numbers) and activity (based on nifH gene expression and the absence of detectable nitrogen fixation rates). In contrast, the mostly autotrophic Pocilloporidae exhibited nifH gene copy numbers and gene expression two orders of magnitude higher than in the Fungiidae, which coincided with detectable nitrogen fixation activity. Based on these data, we suggest that nitrogen fixation compensates for the low heterotrophic nitrogen uptake in autotrophic corals. Consequently, the ecological importance of diazotrophy in coral holobionts may be determined by the trophic functional group of the host.
CitationPogoreutz C, Rädecker N, Cárdenas A, Gärdes A, Wild C, et al. (2017) Nitrogen Fixation Aligns with nifH Abundance and Expression in Two Coral Trophic Functional Groups. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.01187.
SponsorsThe authors thank A.M. Al-Suwailem and Z. Batang for allocation of workspace and P. Muller for technical support at the Core Lab for Coastal and Marine Resources (KAUST). We thank R. Aljadahli, H. Aljadahli, and D. Pallett for support with marine operations, T. Röthig and M. Ziegler for assistance with coral collection, B. Hoeksema for taxonomic clarifications, and Zeyad al-Tallah and N. Karbathia for support with ethylene measurements. Further, the authors would like to thank the editor as well as the two reviewers for their constructive feedback on the manuscript. CP was supported by GLOMAR – Bremen International Graduate School for Marine Sciences. NR was supported by the DAAD Promos Scholarship. CV acknowledges funding by the King Abdullah University of Science and Technology (KAUST). This work was also supported by German Research Foundation (DFG) grant Wi 2677/9-1 to CW.
PublisherFrontiers Media SA
JournalFrontiers in Microbiology
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