Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Online Publication Date2017-06-28
Print Publication Date2017-09
Permanent link to this recordhttp://hdl.handle.net/10754/625159
MetadataShow full item record
AbstractPiriformospora indica is an endophytic fungus colonizing roots of a wide variety of plants. Previous studies showed that P. indica promotes early flowering and plant growth in the medicinal plant Coleus forskohlii. To determine the impact of P. indica on flowering time in Arabidopsis, we co-cultivated the plants with P. indica under long day condition. P. indica inoculated Arabidopsis plants displayed significant early flowering phenotype. qRT-PCR analysis of colonized plants revealed an up-regulation of flowering regulatory (FLOWERING LOCUS T, LEAFY, and APETALA1) and gibberellin biosynthetic (Gibberellin 20-Oxidase2, Gibberellin 3-Oxidase1 and Gibberellin requiring1) genes, while the flowering-repressing gene FLOWERING LOCUS C was down regulated. Quantification of gibberellins content showed that the colonization with P. indica caused an increase in GA4 content. Compared to wild-type plants, inoculation of the Arabidopsis ga5 mutant affected in gibberellin biosynthetic gene led to less pronounced changes in the expression of genes regulating flowering and to a lower increase in GA4 content. Taken together, our data indicate that P. indica promotes early flowering in Arabidopsis likely by increasing gibberellin content.
CitationKim D, Abdelaziz ME, Ntui VO, Guo X, Al-Babili S (2017) Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis. Biochemical and Biophysical Research Communications. Available: http://dx.doi.org/10.1016/j.bbrc.2017.06.169.
SponsorsThis work was funded by King Abdullah University of Science and Technology (KAUST).
- Piriformospora indica promotes early flowering in Arabidopsis through regulation of the photoperiod and gibberellin pathways.
- Authors: Pan R, Xu L, Wei Q, Wu C, Tang W, Oelmüller R, Zhang W
- Issue date: 2017
- The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na(+)/K(+) homeostasis under salt stress conditions.
- Authors: Abdelaziz ME, Kim D, Ali S, Fedoroff NV, Al-Babili S
- Issue date: 2017 Oct
- The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant, Coleus forskohlii.
- Authors: Das A, Kamal S, Shakil NA, Sherameti I, Oelmüller R, Dua M, Tuteja N, Johri AK, Varma A
- Issue date: 2012 Jan
- Root colonization by the endophytic fungus Piriformospora indica shortens the juvenile phase of Piper nigrum L. by fine tuning the floral promotion pathways.
- Authors: Lekshmi RS, Sora S, Anith KN, Soniya EV
- Issue date: 2022
- PYK10, a beta-glucosidase located in the endoplasmatic reticulum, is crucial for the beneficial interaction between Arabidopsis thaliana and the endophytic fungus Piriformospora indica.
- Authors: Sherameti I, Venus Y, Drzewiecki C, Tripathi S, Dan VM, Nitz I, Varma A, Grundler FM, Oelmüller R
- Issue date: 2008 May