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    Fluorescent multiplex cell flow systems and methods

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    WO2017089888A1.pdf
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    Description:
    Published Application
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    Type
    Patent
    Patent Status
    Published Application
    Authors
    Merzaban, Jasmeen cc
    Abuelela, Ayman F.
    Mohammad, Amal Jehad
    Assignee
    King Abdullah University Of Science And Technology
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2017-06-01
    Submitted Date
    2015-11-29
    Permanent link to this record
    http://hdl.handle.net/10754/625141
    
    Metadata
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    Abstract
    Systems and methods are provided for simultaneously assaying cell adhesion or cell rolling for multiple cell specimens. One embodiment provides a system for assaying adhesion or cell rolling of multiple cell specimens that includes a confocal imaging system containing a parallel plate flow chamber, a pump in fluid communication with the parallel plate flow chamber via a flow chamber inlet line and a cell suspension in fluid communication with the parallel plate flow chamber via a flow chamber outlet line. The system also includes a laser scanning system in electronic communication with the confocal imaging system, and a computer in communication with the confocal imaging system and laser scanning system. In certain embodiments, the laser scanning system emits multiple electromagnetic wavelengths simultaneously it cause multiple fluorescent labels having different excitation wavelength maximums to fluoresce. The system can simultaneously capture real-time fluorescence images from at least seven cell specimens in the parallel plate flow chamber.
    Application Number
    WO 2017089888 A1
    Additional Links
    http://www.google.com/patents/WO2017089888A1
    http://worldwide.espacenet.com/publicationDetails/biblio?CC=WO&NR=2017089888A1&KC=A1&FT=D
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Patents

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