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dc.contributor.authorHajjar, Dina A.
dc.contributor.authorKremb, Stephan Georg
dc.contributor.authorSioud, Salim
dc.contributor.authorEmwas, Abdul-Hamid M.
dc.contributor.authorVoolstra, Christian R.
dc.contributor.authorRavasi, Timothy
dc.date.accessioned2017-06-19T09:21:45Z
dc.date.available2017-06-19T09:21:45Z
dc.date.issued2017-06-13
dc.identifier.citationHajjar D, Kremb S, Sioud S, Emwas A-H, Voolstra CR, et al. (2017) Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging. PLOS ONE 12: e0177316. Available: http://dx.doi.org/10.1371/journal.pone.0177316.
dc.identifier.issn1932-6203
dc.identifier.pmid28609451
dc.identifier.doi10.1371/journal.pone.0177316
dc.identifier.urihttp://hdl.handle.net/10754/625051
dc.description.abstractNatural products have been used for medical applications since ancient times. Commonly, natural products are structurally complex chemical compounds that efficiently interact with their biological targets, making them useful drug candidates in cancer therapy. Here, we used cell-based phenotypic profiling and image-based high-content screening to study the mode of action and potential cellular targets of plants historically used in Saudi Arabia's traditional medicine. We compared the cytological profiles of fractions taken from Juniperus phoenicea (Arar), Anastatica hierochuntica (Kaff Maryam), and Citrullus colocynthis (Hanzal) with a set of reference compounds with established modes of action. Cluster analyses of the cytological profiles of the tested compounds suggested that these plants contain possible topoisomerase inhibitors that could be effective in cancer treatment. Using histone H2AX phosphorylation as a marker for DNA damage, we discovered that some of the compounds induced double-strand DNA breaks. Furthermore, chemical analysis of the active fraction isolated from Juniperus phoenicea revealed possible anti-cancer compounds. Our results demonstrate the usefulness of cell-based phenotypic screening of natural products to reveal their biological activities.
dc.description.sponsorshipWe thank Najeh Kharbatia, staff scientist, King Abdullah of Science and Technology (KAUST) Analytical Core Lab. We also thank Dr. Amal Aldhebiani (Assistant Professor of Plant Taxonomy in the Biological Sciences Department at King Abdulaziz University, Jeddah Saudi Arabia) for taxonomic plant identifications. The project was funded by: King Abdullah of Science and Technology (KAUST).
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177316
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleAnti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging
dc.typeArticle
dc.contributor.departmentAnalytical Chemistry Core Lab
dc.contributor.departmentAnalytical Core Lab
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical and Biological Engineering Program
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentKAUST Environmental Epigenetics Research Program (KEEP)
dc.contributor.departmentMarine Science Program
dc.contributor.departmentNMR
dc.contributor.departmentOrganics
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalPLOS ONE
dc.eprint.versionPublisher's Version/PDF
kaust.personHajjar, Dina
kaust.personKremb, Stephan Georg
kaust.personSioud, Salim
kaust.personEmwas, Abdul-Hamid M.
kaust.personVoolstra, Christian R.
kaust.personRavasi, Timothy
refterms.dateFOA2018-06-13T18:43:56Z


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.