Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape
Type
ArticleAuthors
Alamoudi, Kholod
Martins, Patricia
Croissant, Jonas G.
Patil, Sachin

Omar, Haneen

Khashab, Niveen M.

KAUST Department
Advanced Membranes and Porous Materials Research CenterBioscience Program
Chemical Science Program
Physical Science and Engineering (PSE) Division
Smart Hybrid Materials (SHMs) lab
Water Desalination and Reuse Research Center (WDRC)
Date
2017-05-19Online Publication Date
2017-05-19Print Publication Date
2017-06Permanent link to this record
http://hdl.handle.net/10754/625030
Metadata
Show full item recordAbstract
Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.Citation
Alamoudi K, Martins P, Croissant JG, Patil S, Omar H, et al. (2017) Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape. Nanomedicine. Available: http://dx.doi.org/10.2217/nnm-2017-0021.Sponsors
We gratefully acknowledge support from King Abdullah University of Science and Technology (KAUST). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.Publisher
Future Medicine LtdJournal
NanomedicinePubMed ID
28524721Additional Links
http://www.futuremedicine.com/doi/10.2217/nnm-2017-0021ae974a485f413a2113503eed53cd6c53
10.2217/nnm-2017-0021
Scopus Count
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