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dc.contributor.authorLatrasse, David
dc.contributor.authorRodriguez-Granados, Natalia Y.
dc.contributor.authorVeluchamy, Alaguraj
dc.contributor.authorMariappan, Kiruthiga
dc.contributor.authorBevilacqua, Claudia
dc.contributor.authorCrapart, Nicolas
dc.contributor.authorCamps, Celine
dc.contributor.authorSommard, Vivien
dc.contributor.authorRaynaud, Cécile
dc.contributor.authorDogimont, Catherine
dc.contributor.authorBoualem, Adnane
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorBendahmane, Abdelhafid
dc.date.accessioned2017-06-08T09:42:44Z
dc.date.available2017-06-08T09:42:44Z
dc.date.issued2017-05-08
dc.identifier.citationLatrasse D, Rodriguez-Granados NY, Veluchamy A, Mariappan KG, Bevilacqua C, et al. (2017) The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo. Epigenetics & Chromatin 10. Available: http://dx.doi.org/10.1186/s13072-017-0132-6.
dc.identifier.issn1756-8935
dc.identifier.pmid28592995
dc.identifier.doi10.1186/s13072-017-0132-6
dc.identifier.urihttp://hdl.handle.net/10754/624888
dc.description.abstractBackgroundMelon (Cucumis melo) is an important vegetable crop from the Cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. Nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown.ResultsWe have investigated genome wide H3K27me3 and H3K9ac histone modifications and gene expression dynamics, in five melon organs. H3K9ac and H3K27me3 were mainly distributed along gene-rich regions and constrained to gene bodies. H3K9ac was preferentially located at the TSS, whereas H3K27me3 distributed uniformly from TSS to TES. As observed in other species, H3K9ac and H3K27me3 correlated with high and low gene expression levels, respectively. Comparative analyses of unisexual flowers pointed out sex-specific epigenetic states of TFs involved in ethylene response and flower development. Chip-qPCR analysis of laser dissected carpel and stamina primordia, revealed sex-specific histone modification of MADS-box genes. Using sex transition mutants, we demonstrated that the female promoting gene, CmACS11, represses the expression of the male promoting gene CmWIP1 via deposition of H3K27me3.ConclusionsOur findings reveal the organ-specific landscapes of H3K9ac and H3K27me3 in melon. Our results also provide evidence that the sex determination genes recruit histone modifiers to orchestrate unisexual flower development in monoecious species.
dc.description.sponsorshipThis work was supported by the Plant Biology and Breeding department in INRA, the grants Program Saclay Plant Sciences (SPS, ANR-10-LABX-40), Inititiative d’Excellence Paris-Saclay (Lidex-3P, ANR-11-IDEX-0003-02), L’Agence Nationale de la Recherche MELODY (ANR-11-BSV7-0024), and the European Research Council (ERC-SEXYPARTH).
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/article/10.1186/s13072-017-0132-6
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe quest for epigenetic regulation underlying unisexual flower development in Cucumis melo
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.identifier.journalEpigenetics & Chromatin
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Plant Sciences Paris‑Saclay (IPS2), CNRS, INRA, University Paris‑Sud, University of Evry, University Paris‑Diderot, Sorbonne Paris‑Cite, University of Paris-Saclay, Batiment 630, 91405 Orsay, France.
dc.contributor.institutionUMR 1313 Génétique Animale et Biologie Intégrative, Institut National de la Recherche Agronomique, 78350 Jouy‑en‑Josas, France.
dc.contributor.institutionUR 1052, Unité de Génétique et d’Amélioration des Fruits et Légumes, INRA, BP94, 84143 Montfavet, France.
kaust.personVeluchamy, Alaguraj
kaust.personMariappan, Kiruthiga
kaust.personBenhamed, Moussa
refterms.dateFOA2018-06-13T15:16:36Z


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.