Data from: Using a butterflyfish genome as a general tool for RAD-Seq studies in specialized reef fish
Saenz Agudelo, Pablo
Piatek, Marek J.
Berumen, Michael L.
KAUST DepartmentRed Sea Research Center (RSRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/624180
MetadataShow full item record
CitationDiBattista, J. D., Saenz-Agudelo, P., Piatek, M. J., Wang, X., Aranda, M., & Berumen, M. L. (2017). Data from: Using a butterflyfish genome as a general tool for RAD-Seq studies in specialized reef fish (Version 2) [Data set]. Dryad Digital Repository. https://doi.org/10.5061/dryad.f09rh
PublisherDryad Digital Repository
Except where otherwise noted, this item's license is described as http://creativecommons.org/publicdomain/zero/1.0/
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External tagging does not affect the feeding behavior of a coral reef fish, Chaetodon vagabundus (Pisces: Chaetodontidae)Berumen, Michael L.; Almany, Glenn R. (Environmental Biology of Fishes, Springer Nature, 2009-11-10) [Article]Increasingly, the ability to recognize individual fishes is important for studies of population dynamics, ecology, and behavior. Although a variety of methods exist, external tags remain one of the most widely applied because they are both effective and cost efficient. However, a key assumption is that neither the tagging procedure nor the presence of a tag negatively affects the individual. While this has been demonstrated for relatively coarse metrics such as growth and survival, few studies have examined the impact of tags and tagging on more subtle aspects of behavior. We tagged adult vagabond butterflyfish (Chaetodon vagabundus) occupying a 30-ha insular reef in Kimbe Bay, Papua New Guinea, using a commonly-utilized t-bar anchor tag. We quantified and compared feeding behavior (bite rate), which is sensitive to stress, of tagged and untagged individuals over four separate sampling periods spanning 4 months post-tagging. Bite rates did not differ between tagged and untagged individuals at each sampling period and, combined with additional anecdotal observations of normal pairing behavior and successful reproduction, suggest that tagging did not adversely affect individuals. © Springer Science+Business Media B.V. 2009.
Blinded by the bright: a lack of congruence between colour morphs, phylogeography and taxonomy for a cosmopolitan Indo-Pacific butterflyfish, Chaetodon aurigaDiBattista, Joseph; Waldrop, Ellen; Rocha, Luiz A.; Craig, Matthew T.; Berumen, Michael L.; Bowen, Brian W. (Journal of Biogeography, Wiley-Blackwell, 2015-07) [Article]Aim: We assess genetic differentiation among biogeographical provinces and colour morphs of the threadfin butterflyfish, Chaetodon auriga. This species is among the most broadly distributed butterflyfishes in the world, occurring on reefs from the Red Sea and western Indian Ocean to French Polynesia and Hawai'i. The Red Sea form lacks a conspicuous 'eye-spot' on the dorsal fin, which may indicate an evolutionary distinction. Location: Red Sea, Indian Ocean and Pacific Ocean. Methods: Specimens were obtained at 17 locations (n = 358) spanning the entire range of this species. The genetic data included 669 base pairs of mitochondrial DNA (mtDNA) cytochrome b and allele frequencies at six microsatellite loci. Analysis of molecular variance, structure plots, haplotype networks and estimates of population expansion time were used to assess phylogeographical patterns. Results: Population structure was low overall, but significant and concordant between molecular markers (mtDNA: ΦST = 0.027, P < 0.001; microsatellites: FST = 0.023, P < 0.001). Significant population-level partitions were only detected at peripheral locations including the Red Sea and Hawai'i. Population expansion events in the Red Sea and Socotra are older (111,940-223,881 years) relative to all other sites (16,343-87,910 years). Main conclusions: We find little genetic evidence to support an evolutionary partition of a previously proposed Red Sea subspecies. The oldest estimate of population expansion in the Red Sea and adjacent Gulf of Aden indicates a putative refuge in this region during Pleistocene glacial cycles. The finding of population separations at the limits of the range, in the Red Sea and Hawai'i, is consistent with peripheral speciation. © 2015 John Wiley & Sons Ltd.
Characterization of 11 novel microsatellite markers for the vagabond butterflyfish, Chaetodon vagabundusSaenz Agudelo, Pablo; Almany, Glenn R.; Mansour, Hicham; Perumal, Sadhasivam; Berumen, Michael L. (Conservation Genetics Resources, Springer Science + Business Media, 2015-02-21) [Article]Microsatellite markers were developed for the coral reef fish Chaetodon vagabundus using shotgun pyrosequencing. As threats to coral reefs intensify, information on larval connectivity is of increasing value for efficient conservation planning. Here, 11 novel microsatellites were characterized for 192 individuals from Papua New Guinea. The number of alleles per locus ranged from 7 to 32, while observed and expected heterozygosity values varied from 0.214 to 0.903. These markers will be used to study population structure and larval connectivity of this iconic coral reef fish in coral reefs across the Indo-Pacific.