Show simple item record

dc.contributor.authorAlshareef, Sahar
dc.contributor.authorLing, Yu
dc.contributor.authorButt, Haroon
dc.contributor.authorMariappan, Kiruthiga
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2017-06-06T07:44:34Z
dc.date.available2017-06-06T07:44:34Z
dc.date.created2017-03-28
dc.date.issued2017
dc.identifier.citationAlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597
dc.identifier.doi10.6084/m9.figshare.c.3727597
dc.identifier.urihttp://hdl.handle.net/10754/624149
dc.description.abstractAbstract Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.
dc.publisherFigshare
dc.rightsCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiophysics
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectGenetics
dc.subjectMolecular Biology
dc.subjectEcology
dc.subjectDevelopmental Biology
dc.titleSupplementary Material for: Herboxidiene triggers splicing repression and abiotic stress responses in plants
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
kaust.personAlshareef, Sahar
kaust.personLing, Yu
kaust.personButt, Haroon
kaust.personMariappan, Kiruthiga
kaust.personBenhamed, Moussa
dc.type.resourceCollection
dc.relation.isSupplementToAlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.
dc.relation.isSupplementToDOI:10.1186/s12864-017-3656-z
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/623088


This item appears in the following Collection(s)

Show simple item record

CC BY
Except where otherwise noted, this item's license is described as CC BY