Supplementary Material for: Herboxidiene triggers splicing repression and abiotic stress responses in plants
Type
DatasetAuthors
Alshareef, Sahar
Ling, Yu

Butt, Haroon
Mariappan, Kiruthiga

Benhamed, Moussa

Mahfouz, Magdy M.

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Desert Agriculture Initiative
Lab Consumables & Gases
Plant Science
Plant Science Program
Purchasing
Date
2017Permanent link to this record
http://hdl.handle.net/10754/624149
Metadata
Show full item recordAbstract
Abstract Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.Citation
AlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597Publisher
figshareRelations
Is Supplement To:- [Article]
AlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.. DOI: 10.1186/s12864-017-3656-z HANDLE: 10754/623088
ae974a485f413a2113503eed53cd6c53
10.6084/m9.figshare.c.3727597