Supplementary Material for: CRISPR/Cas9-mediated viral interference in plants
Type
DatasetKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Date
2015Permanent link to this record
http://hdl.handle.net/10754/624131
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Abstract Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.Citation
Ali, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878Publisher
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Is Supplement To:- [Article]
CRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology. DOI: 10.1186/s13059-015-0799-6 HANDLE: 10754/582543
ae974a485f413a2113503eed53cd6c53
10.6084/m9.figshare.c.3612878