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dc.contributor.authorGhanimeh, Sophia
dc.contributor.authorEl-Fadel, Mutasem
dc.contributor.authorSaikaly, Pascal
dc.date.accessioned2017-06-05T06:02:24Z
dc.date.available2017-06-05T06:02:24Z
dc.date.issued2017-07-19
dc.identifier.citationGhanimeh S, El-Fadel M, Saikaly PE (2017) Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity. Journal of Chemical Technology & Biotechnology. Available: http://dx.doi.org/10.1002/jctb.5341.
dc.identifier.issn0268-2575
dc.identifier.doi10.1002/jctb.5341
dc.identifier.urihttp://hdl.handle.net/10754/624042
dc.description.abstractBACKGROUND Reportedly, various mixes of seeds were quasi-randomly selected to startup anaerobic digesters. In contrast, this study examines the impact of inoculating thermophilic anaerobic digesters with a designed mix of non-acclimated seeds based on their methanogen composition, using Quantitative Polymerase Chain Reaction (QPCR) of 16S rRNA gene, to achieve high abundance and diversity of methanogens. RESULTS Based on QPCR results, two seed mixes were selected to inoculate two anaerobic digesters: digester (A) was inoculated with a control seed consisting of digestate, manure, and activated sludge; and digester (B) was inoculated with a further methanogen-enriched seed consisting of the control seed with added compost and leachate. Both seed combinations yielded a balanced microflora that is able to achieve a successful startup. However, upon reaching steady state, digester B exhibited lower propionate levels, resulting in lower VFA concentration and increased buffering capacity, indicating greater stability. Acetotrophs and hydrogenotrophs were dominated by Methanosarcinaceae and Methanobacteriales, respectively, in both digesters, exhibiting an average ratio of 66-to-34% in A and 76-to-24% in B during steady state. CONCLUSION The inoculation strategy in digester B resulted in improved stability, lower propionate concentration and 10% higher relative abundance of acetotrophs.
dc.description.sponsorshipThis study was supported by the National Council for Scientific Research-Lebanon, and the Masri Institute of Energy and Natural Resources at the American University of Beirut. The experimental setup was obtained through a grant from the US Agency for International Development. Special thanks are extended to Mr. Khaled Salam for his assistance in performing the QPCR analysis.
dc.publisherWiley
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jctb.5341/abstract
dc.rightsThis is the peer reviewed version of the following article: Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity, which has been published in final form at http://doi.org/10.1002/jctb.5341. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
dc.subjectAnaerobic digestion
dc.subjectBiomass
dc.subjectWaste treatment and minimization
dc.titlePerformance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalJournal of Chemical Technology & Biotechnology
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Civil and Environmental Engineering; American University of Beirut
dc.contributor.institutionDepartment of Civil and Environmental Engineering; Notre Dame University-Louaize
kaust.personSaikaly, Pascal
refterms.dateFOA2018-05-29T00:00:00Z
dc.date.published-online2017-07-19
dc.date.published-print2018-01


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