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    RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes

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    Type
    Article
    Authors
    Lee, Keh Chien
    Jang, Yun Hee
    Kim, SoonKap
    Park, Hyo-Young
    Thu, May Phyo
    Lee, Jeong Hwan
    Kim, Jeong-Kook
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Date
    2017-04-11
    Online Publication Date
    2017-04-11
    Print Publication Date
    2017-07
    Permanent link to this record
    http://hdl.handle.net/10754/623904
    
    Metadata
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    Abstract
    The RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing of FLOWERING LOCUS M pre-mRNA and a heat shock transcription factor HsfA2 pre-mRNA. Splicing factor 1 (SF1) plays a crucial role in 3' splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions. For example, the deletion of RRM domain influences AtSF1-mediated control of flowering time, but not the abscisic acid sensitivity response during seed germination. The alternative splicing of FLOWERING LOCUS M (FLM) pre-mRNA is involved in flowering time control. We found that the RRM domain of AtSF1 protein alters the production of alternatively spliced FLM-β transcripts. We also found that the RRM domain affects the alternative splicing of a heat shock transcription factor HsfA2 pre-mRNA, thereby mediating the heat stress response. Taken together, our results suggest the importance of RRM domain for AtSF1-mediated alternative splicing of a subset of genes involved in the regulation of flowering and adaptation to heat stress.
    Citation
    Lee KC, Jang YH, Kim S-K, Park H-Y, Thu MP, et al. (2017) RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes. Plant Cell Reports. Available: http://dx.doi.org/10.1007/s00299-017-2140-1.
    Sponsors
    This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2014R1A1A2055796 to J.-K. Kim) and a Korea University Grant (to J.-K. Kim).
    Publisher
    Springer Nature
    Journal
    Plant Cell Reports
    DOI
    10.1007/s00299-017-2140-1
    Additional Links
    http://link.springer.com/article/10.1007%2Fs00299-017-2140-1
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00299-017-2140-1
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Center for Desert Agriculture

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