• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Nagymihály, Marianna
    Veluchamy, Alaguraj cc
    Györgypál, Zoltán
    Ariel, Federico
    Jégu, Teddy
    Benhamed, Moussa cc
    Szűcs, Attila
    Kereszt, Attila cc
    Mergaert, Peter
    Kondorosi, Éva
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2017-04-12
    Online Publication Date
    2017-04-12
    Print Publication Date
    2017-04-25
    Permanent link to this record
    http://hdl.handle.net/10754/623891
    
    Metadata
    Show full item record
    Abstract
    The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplication-dependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.
    Citation
    Nagymihály M, Veluchamy A, Györgypál Z, Ariel F, Jégu T, et al. (2017) Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression. Proceedings of the National Academy of Sciences 114: 4543–4548. Available: http://dx.doi.org/10.1073/pnas.1704211114.
    Sponsors
    We thank Célia Casset for help with the qPCR experiments, Dr. Mickael Bourge for the flow cytometry sorting of nodule nuclei, and Dr. István Nagy for providing the MBD2 protein for the MeDIP experiment. This work benefitted from the facilities and expertise of the Imagif Cell Biology Unit of the Gif campus (which is supported by the Infrastructures en Biologie Santé et Agronomie), the Agence Nationale de la Recherche (ANR) under Investments for the Future programs France-BioImaging Infrastructure Grant ANR-10-INSB-04-01 and Saclay Plant Sciences Grant ANR-10-LABX-0040-SPS, and the Conseil Général de l’Essonne. This work was supported by ANR Grant ANR-13-BSV7-0013 and by European Research Council SYM-BIOTICS Advanced Grant 269067 (to É.K.). M.N. was the recipient of a PhD grant from CAMPUS France and was supported by the SYM-BIOTICS grant.
    Publisher
    Proceedings of the National Academy of Sciences
    Journal
    Proceedings of the National Academy of Sciences
    DOI
    10.1073/pnas.1704211114
    Additional Links
    http://www.pnas.org/content/early/2017/04/11/1704211114.full
    ae974a485f413a2113503eed53cd6c53
    10.1073/pnas.1704211114
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.