Show simple item record

dc.contributor.authorMarini, Monica
dc.contributor.authorLimongi, Tania
dc.contributor.authorFalqui, Andrea
dc.contributor.authorGenovese, Alessandro
dc.contributor.authorAllione, Marco
dc.contributor.authorMoretti, Manola
dc.contributor.authorLopatin, Sergei
dc.contributor.authorTirinato, Luca
dc.contributor.authorDas, Gobind
dc.contributor.authorTorre, Bruno
dc.contributor.authorGiugni, Andrea
dc.contributor.authorCesca, F.
dc.contributor.authorBenfenati, F.
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2017-05-31T11:23:09Z
dc.date.available2017-05-31T11:23:09Z
dc.date.issued2017-01-17
dc.identifier.citationMarini M, Limongi T, Falqui A, Genovese A, Allione M, et al. (2017) Imaging and structural studies of DNA–protein complexes and membrane ion channels. Nanoscale 9: 2768–2777. Available: http://dx.doi.org/10.1039/c6nr07958j.
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.doi10.1039/c6nr07958j
dc.identifier.urihttp://hdl.handle.net/10754/623846
dc.description.abstractIn bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABA(A) channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 angstrom, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from the Italian Ministry of Health under project no. GR-2010-2320665 and GR-2010-2311677.
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C6NR07958J#!divAbstract
dc.rightsArchived with thanks to Nanoscale
dc.titleImaging and structural studies of DNA–protein complexes and membrane ion channels
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentMaterials Science and Engineering Program
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Division
dc.identifier.journalNanoscale
dc.eprint.versionPost-print
dc.contributor.institutionCenter for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genova, Italy
kaust.personMarini, Monica
kaust.personLimongi, Tania
kaust.personFalqui, Andrea
kaust.personGenovese, Alessandro
kaust.personAllione, Marco
kaust.personMoretti, Manola
kaust.personLopatin, Sergei
kaust.personTirinato, Luca
kaust.personDas, Gobind
kaust.personTorre, Bruno
kaust.personGiugni, Andrea
kaust.personDi Fabrizio, Enzo M.
refterms.dateFOA2018-01-17T00:00:00Z


Files in this item

Thumbnail
Name:
c6nr07958j.pdf
Size:
6.154Mb
Format:
PDF
Description:
Accepted Manuscript

This item appears in the following Collection(s)

Show simple item record