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dc.contributor.authorHurbain, Ilse
dc.contributor.authorRomao, Maryse
dc.contributor.authorBergam, Ptissam
dc.contributor.authorHeiligenstein, Xavier
dc.contributor.authorRaposo, Graça
dc.date.accessioned2017-05-31T11:23:05Z
dc.date.available2017-05-31T11:23:05Z
dc.date.issued2017-04-30
dc.identifier.citationHurbain I, Romao M, Bergam P, Heiligenstein X, Raposo G (2017) Analyzing Lysosome-Related Organelles by Electron Microscopy. Lysosomes: 43–71. Available: http://dx.doi.org/10.1007/978-1-4939-6934-0_4.
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.doi10.1007/978-1-4939-6934-0_4
dc.identifier.urihttp://hdl.handle.net/10754/623792
dc.description.abstractIntracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
dc.description.sponsorshipWe are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4
dc.subjectLysosome-related organelles
dc.subjectTransmission electron microscopy
dc.subjectChemical fixation
dc.subjectHigh pressure freezing
dc.subjectFreeze substitution
dc.subjectImmunolabeling
dc.subjectTokuyasu
dc.titleAnalyzing Lysosome-Related Organelles by Electron Microscopy
dc.typeBook Chapter
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.identifier.journalMethods in Molecular Biology
dc.contributor.institutionInstitut Curie, PSL Research University, CNRS, UMR 144, F-75005, Paris, France
dc.contributor.institutionSorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 144, F-75005, Paris, France
dc.contributor.institutionCell and Tissue Imaging Core Facility PICT-IBiSA, Institut Curie, F-75248, Paris, France
kaust.personBergam, Ptissam
dc.date.published-online2017-04-30
dc.date.published-print2017


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