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    Analyzing Lysosome-Related Organelles by Electron Microscopy

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    Type
    Book Chapter
    Authors
    Hurbain, Ilse
    Romao, Maryse
    Bergam, Ptissam
    Heiligenstein, Xavier
    Raposo, Graça
    KAUST Department
    Electron Microscopy
    Imaging and Characterization Core Lab
    Date
    2017-04-30
    Online Publication Date
    2017-04-30
    Print Publication Date
    2017
    Permanent link to this record
    http://hdl.handle.net/10754/623792
    
    Metadata
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    Abstract
    Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
    Citation
    Hurbain I, Romao M, Bergam P, Heiligenstein X, Raposo G (2017) Analyzing Lysosome-Related Organelles by Electron Microscopy. Lysosomes: 43–71. Available: http://dx.doi.org/10.1007/978-1-4939-6934-0_4.
    Sponsors
    We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.
    Publisher
    Springer Nature
    Journal
    Methods in Molecular Biology
    DOI
    10.1007/978-1-4939-6934-0_4
    Additional Links
    http://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-4939-6934-0_4
    Scopus Count
    Collections
    Imaging and Characterization Core Lab; Book Chapters

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