Type
Book ChapterKAUST Department
Electron MicroscopyImaging and Characterization Core Lab
Date
2017-04-30Online Publication Date
2017-04-30Print Publication Date
2017Permanent link to this record
http://hdl.handle.net/10754/623792
Metadata
Show full item recordAbstract
Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.Citation
Hurbain I, Romao M, Bergam P, Heiligenstein X, Raposo G (2017) Analyzing Lysosome-Related Organelles by Electron Microscopy. Lysosomes: 43–71. Available: http://dx.doi.org/10.1007/978-1-4939-6934-0_4.Sponsors
We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.Publisher
Springer NatureJournal
Methods in Molecular BiologyAdditional Links
http://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4ae974a485f413a2113503eed53cd6c53
10.1007/978-1-4939-6934-0_4