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dc.contributor.advisorPain, Arnab
dc.contributor.authorDouvropoulou, Olga
dc.date.accessioned2017-05-28T05:30:35Z
dc.date.available2018-05-25T00:00:00Z
dc.date.issued2017-04
dc.identifier.citationDouvropoulou, O. (2017). Interactions between Cryptosporidium parvum and the Intestinal Ecosystem. KAUST Research Repository. https://doi.org/10.25781/KAUST-E49CT
dc.identifier.doi10.25781/KAUST-E49CT
dc.identifier.urihttp://hdl.handle.net/10754/623721
dc.description.abstractCryptosporidium parvum is an apicomplexan protozoan parasite commonly causing diarrhea, particularly in infants in developing countries. The research challenges faced in the development of therapies against Cryptosporidium slow down the process of drug discovery. However, advancement of knowledge towards the interactions of the intestinal ecosystem and the parasite could provide alternative approaches to tackle the disease. Under this perspective, the primary focus of this work was to study interactions between Cryptosporidium parvum and the intestinal ecosystem in a mouse model. Mice were treated with antibiotics with different activity spectra and the resulted perturbation of the native gut microbiota was identified by microbiome studies. In particular, 16S amplicon sequencing and Whole Genome Sequencing (WGS) were used to determine the bacterial composition and the genetic repertoire of the fecal microbial communities in the mouse gut. Following alteration of the microbial communities of mice by application of antibiotic treatment, Cryptosporidium parasites were propagated in mice with perturbed microbiota and the severity of the infection was quantified. This approach enabled the prediction of the functional capacity of the microbial communities in the mouse gut and led to the identification of bacterial taxa that positively or negatively correlate in abundance with Cryptosporidium proliferation.
dc.language.isoen
dc.subjectCryptosporidium
dc.subjectmicrobiota
dc.subjectdysbiosis
dc.titleInteractions between Cryptosporidium parvum and the Intestinal Ecosystem
dc.typeThesis
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.rights.embargodate2018-05-25
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberGojobori, Takashi
dc.contributor.committeememberHong, Pei-Ying
dc.contributor.committeememberWidmer, Giovanni
thesis.degree.disciplineBioscience
thesis.degree.nameMaster of Science
dc.rights.accessrightsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2018-05-25.
refterms.dateFOA2018-05-25T00:00:00Z


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