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dc.contributor.authorGuan, Yue
dc.contributor.authorHaroon, Mohamed
dc.contributor.authorAlam, Intikhab
dc.contributor.authorFerry, James G.
dc.contributor.authorStingl, Ulrich
dc.date.accessioned2017-05-17T07:41:40Z
dc.date.available2017-05-17T07:41:40Z
dc.date.issued2017-05-11
dc.identifier.citationGuan Y, Haroon MF, Alam I, Ferry JG, Stingl U (2017) Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12545.
dc.identifier.issn1758-2229
dc.identifier.pmid28493460
dc.identifier.doi10.1111/1758-2229.12545
dc.identifier.urihttp://hdl.handle.net/10754/623647
dc.description.abstractPyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.
dc.description.sponsorshipThis study was supported by King Abdullah University of Science and Technology (KAUST) through baseline funding and the SEDCO Research Excellence award to US.
dc.publisherWiley-Blackwell
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12545/abstract
dc.rightsThis is the peer reviewed version of the following article: Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1, which has been published in final form at http://doi.org/10.1111/1758-2229.12545. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
dc.titleSingle-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalEnvironmental Microbiology Reports
dc.eprint.versionPost-print
dc.contributor.institutionHarvard University, Department of Organismic and Evolutionary Biology; Cambridge MA 02138 USA
dc.contributor.institutionPennsylvania State University, Department of Biochemistry and Molecular Biology; University Park PA 16802 USA
dc.contributor.institutionUniversity of Florida/IFAS, Department for Microbiology & Cell Science, Fort Lauderdale Research and Education Center; Davie FL 33314 USA
kaust.personGuan, Yue
kaust.personHaroon, Mohamed
kaust.personAlam, Intikhab
kaust.personStingl, Ulrich
refterms.dateFOA2018-05-10T00:00:00Z


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