Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Marine Science Program
Office of the VP
Red Sea Research Center (RSRC)
Online Publication Date2017-06-06
Print Publication Date2017-08
Permanent link to this recordhttp://hdl.handle.net/10754/623647
MetadataShow full item record
AbstractPyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.
CitationGuan Y, Haroon MF, Alam I, Ferry JG, Stingl U (2017) Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12545.
SponsorsThis study was supported by King Abdullah University of Science and Technology (KAUST) through baseline funding and the SEDCO Research Excellence award to US.
- Pyrrolysine and selenocysteine use dissimilar decoding strategies.
- Authors: Zhang Y, Baranov PV, Atkins JF, Gladyshev VN
- Issue date: 2005 May 27
- Metabolic traits of an uncultured archaeal lineage--MSBL1--from brine pools of the Red Sea.
- Authors: Mwirichia R, Alam I, Rashid M, Vinu M, Ba-Alawi W, Anthony Kamau A, Kamanda Ngugi D, Göker M, Klenk HP, Bajic V, Stingl U
- Issue date: 2016 Jan 13
- A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine.
- Authors: Longstaff DG, Larue RC, Faust JE, Mahapatra A, Zhang L, Green-Church KB, Krzycki JA
- Issue date: 2007 Jan 16
- An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
- Authors: Polycarpo C, Ambrogelly A, Bérubé A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Söll D
- Issue date: 2004 Aug 24
- The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine.
- Authors: Gaston MA, Zhang L, Green-Church KB, Krzycki JA
- Issue date: 2011 Mar 31