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dc.contributor.authorBüchel, Gabriel E.
dc.contributor.authorCarney, Brandon
dc.contributor.authorTang, Jun
dc.contributor.authorZeglis, Brian M.
dc.contributor.authorEppinger, Jörg
dc.contributor.authorReiner, Thomas
dc.date.accessioned2017-05-17T07:41:38Z
dc.date.available2017-05-17T07:41:38Z
dc.date.issued2017-03-09
dc.identifier.citationBüchel GE, Carney B, Tang J, Zeglis BM, Eppinger J, et al. (2017) A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting. Journal of Visualized Experiments. Available: http://dx.doi.org/10.3791/54694.
dc.identifier.issn1940-087X
dc.identifier.doi10.3791/54694
dc.identifier.urihttp://hdl.handle.net/10754/623625
dc.description.abstractIntraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm(2). In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room.
dc.description.sponsorshipThe authors thank Prof. Jan Grimm and Mr. Travis Shaffer for their helpful discussions and Mr. David Gregory for editing the manuscript. Technical services provided by the MSK Animal Imaging Core Facility, supported in part by NIH Cancer Center Support Grant P30CA008748-48, are gratefully acknowledged. The authors thank the NIH (K25 EB016673 and R21 CA191679, T.R. and 4R00CA178205-02, B.M.Z.), the MSK Center for Molecular Imaging and Nanotechnology (T.R.), the Tow Foundation (B.C.), and the National Science Foundation Integrative Graduate Education and Research Traineeship (IGERT 0965983 at Hunter College for B.C. and T.M.S.) for their generous support. The research reported in this publication was supported by funding from the King Abdullah University of Science and Technology.
dc.publisherMyJove Corporation
dc.relation.urlhttps://www.jove.com/video/54694/a-novel-technique-for-generating-observing-chemiluminescence
dc.rightsArchived with thanks to Journal of Visualized Experiments
dc.subjectBioengineering
dc.subjectIssue 121
dc.subjectRuthenium
dc.subjectCerium
dc.subjectChemiluminescence
dc.subjectIntraoperative imaging
dc.subjectLymph nodes
dc.subjectin vivo
dc.titleA Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
dc.typeArticle
dc.contributor.departmentBiological & Organometallic Catalysis Laboratories
dc.contributor.departmentChemical Science Program
dc.contributor.departmentKAUST Catalysis Center (KCC)
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalJournal of Visualized Experiments
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Radiology, Memorial Sloan Kettering Cancer Center, , United States
dc.contributor.institutionDepartment of Chemistry, Hunter College, , United States
dc.contributor.institutionGraduate Center of City University of New York, , United States
dc.contributor.institutionDepartment of Radiology, Weill Cornell Medical College, , United States
kaust.personBüchel, Gabriel E.
kaust.personEppinger, Jorg
refterms.dateFOA2019-03-09T00:00:00Z


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