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dc.contributor.authorZaidi, Syed Shan-e-Ali
dc.contributor.authorTashkandi, Manal
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2017-05-09T12:54:46Z
dc.date.available2017-05-09T12:54:46Z
dc.date.issued2017-04-26
dc.identifier.citationZaidi SS-A, Tashkandi M, Mahfouz MM (2017) Engineering Molecular Immunity Against Plant Viruses. Gene Editing in Plants: 167–186. Available: http://dx.doi.org/10.1016/bs.pmbts.2017.03.009.
dc.identifier.issn1877-1173
dc.identifier.doi10.1016/bs.pmbts.2017.03.009
dc.identifier.urihttp://hdl.handle.net/10754/623451
dc.description.abstractGenomic engineering has been used to precisely alter eukaryotic genomes at the single-base level for targeted gene editing, replacement, fusion, and mutagenesis, and plant viruses such as Tobacco rattle virus have been developed into efficient vectors for delivering genome-engineering reagents. In addition to altering the host genome, these methods can target pathogens to engineer molecular immunity. Indeed, recent studies have shown that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) systems that target the genomes of DNA viruses can interfere with viral activity and limit viral symptoms in planta, demonstrating the utility of this system for engineering molecular immunity in plants. CRISPR/Cas9 can efficiently target single and multiple viral infections and confer plant immunity. Here, we discuss the use of site-specific nucleases to engineer molecular immunity against DNA and RNA viruses in plants. We also explore how to address the potential challenges encountered when producing plants with engineered resistance to single and mixed viral infections.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1877117317300418
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Progress in Molecular Biology and Translational Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Progress in Molecular Biology and Translational Science, [, , (2017-04-26)] DOI: 10.1016/bs.pmbts.2017.03.009. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSite-specific nuclease
dc.subjectGenome engineering
dc.subjectCRISPR/Cas9
dc.subjectGeminiviruses
dc.subjectTobacco rattle virus
dc.titleEngineering Molecular Immunity Against Plant Viruses
dc.typeBook Chapter
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.identifier.journalProgress in Molecular Biology and Translational Science
dc.eprint.versionPost-print
dc.contributor.institutionNational Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
kaust.personZaidi, Syed Shan-e-Ali
kaust.personTashkandi, Manal
kaust.personMahfouz, Magdy M.
refterms.dateFOA2018-06-14T05:03:54Z
dc.date.published-online2017-04-26
dc.date.published-print2017


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