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dc.contributor.authorPark, Hyo-Young
dc.contributor.authorLee, Keh Chien
dc.contributor.authorJang, Yun Hee
dc.contributor.authorKim, SoonKap
dc.contributor.authorThu, May Phyo
dc.contributor.authorLee, Jeong Hwan
dc.contributor.authorKim, Jeong-Kook
dc.date.accessioned2017-05-09T12:54:46Z
dc.date.available2017-05-09T12:54:46Z
dc.date.issued2017-04-21
dc.identifier.citationPark H-Y, Lee KC, Jang YH, Kim S-K, Thu MP, et al. (2017) The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. Plant Cell Reports. Available: http://dx.doi.org/10.1007/s00299-017-2142-z.
dc.identifier.issn0721-7714
dc.identifier.issn1432-203X
dc.identifier.doi10.1007/s00299-017-2142-z
dc.identifier.urihttp://hdl.handle.net/10754/623448
dc.description.abstractThe Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins' Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.
dc.description.sponsorshipThis work was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (to J.-K. Kim), and by a Korea University Grant (to J.-K. Kim).
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs00299-017-2142-z
dc.subjectFRAP
dc.subjectShuttling
dc.subjectFLIP
dc.subjectDynamic Movement
dc.subjectSf1
dc.subjectU2af65
dc.subjectU2af35
dc.titleThe Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalPlant Cell Reports
dc.contributor.institutionDepartment of Life Sciences, Korea University, Anam-dong 5 ga, Seongbuk-gu, Seoul, 02841, Republic of Korea.
dc.contributor.institutionDepartment of Life Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-Si, Jeollabuk-do, 54896, Republic of Korea. jhwanlee90@jbnu.ac.kr.
dc.contributor.institutionDepartment of Life Sciences, Korea University, Anam-dong 5 ga, Seongbuk-gu, Seoul, 02841, Republic of Korea. jkkim@korea.ac.kr.
kaust.personKim, SoonKap
dc.date.published-online2017-04-21
dc.date.published-print2017-07


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