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    The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms

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    Type
    Article
    Authors
    Park, Hyo-Young
    Lee, Keh Chien
    Jang, Yun Hee
    Kim, SoonKap
    Thu, May Phyo
    Lee, Jeong Hwan
    Kim, Jeong-Kook
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Desert Agriculture Initiative
    Date
    2017-04-21
    Online Publication Date
    2017-04-21
    Print Publication Date
    2017-07
    Permanent link to this record
    http://hdl.handle.net/10754/623448
    
    Metadata
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    Abstract
    The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins' Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.
    Citation
    Park H-Y, Lee KC, Jang YH, Kim S-K, Thu MP, et al. (2017) The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. Plant Cell Reports. Available: http://dx.doi.org/10.1007/s00299-017-2142-z.
    Sponsors
    This work was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (to J.-K. Kim), and by a Korea University Grant (to J.-K. Kim).
    Publisher
    Springer Nature
    Journal
    Plant Cell Reports
    DOI
    10.1007/s00299-017-2142-z
    Additional Links
    http://link.springer.com/article/10.1007%2Fs00299-017-2142-z
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00299-017-2142-z
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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