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dc.contributor.advisorMerzaban, Jasmeen
dc.contributor.authorAl-Amoodi, Asma S.
dc.date.accessioned2017-05-08T11:22:30Z
dc.date.available2018-05-07T00:00:00Z
dc.date.issued2017-05
dc.identifier.citationAl-Amoodi, A. S. (2017). Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X. KAUST Research Repository. https://doi.org/10.25781/KAUST-28D97
dc.identifier.doi10.25781/KAUST-28D97
dc.identifier.urihttp://hdl.handle.net/10754/623399
dc.description.abstractRecruitment of circulating cells towards target sites is primarily dependent on E-selectin receptor/ligand adhesive interactions. Glycosyltransferase (GTs) are involved in the creation of E-selectin ligands. A sialofucosylated terminal tetrasaccharide like glycan structure known as sialyl Lewis x (sLex), is the most recognized ligand by selectins. This structure is found on the surface of cancer cells and leukocytes but is often absent on the surface of many adult stem cell populations. In order to synthesize sLex, GTs must be endogenously expressed and remain active within the cells. Generally, these stem cells express terminal sialylated lactosamine structures on their glycoproteins which require the addition of alpha-(1,3)-fucose to be converted into an E-selectin ligand. There are a number of fucosyltransferases (FUTs) that are able to modify terminal lactosamine structures to create sLex such as FUT6. In this work we focused on expressing and purifying active recombinant FUTs as a tool to help create sLex structures on the surface of adult stem cells in order to enhance their migration.
dc.language.isoen
dc.subjectStem Cells
dc.subjectCellular migration
dc.subjectFUTs purification
dc.titleExpression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X
dc.typeThesis
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.rights.embargodate2018-05-07
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberLi, Mo
dc.contributor.committeememberMahfouz, Magdy M.
thesis.degree.disciplineBioscience
thesis.degree.nameMaster of Science
dc.rights.accessrightsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2018-05-07.
refterms.dateFOA2018-05-07T00:00:00Z


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