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    Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X

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    MS Thesis_Asma Alamoodi_Spring 2017.pdf
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    Description:
    MS Thesis_Asma
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    Type
    Thesis
    Authors
    Al-Amoodi, Asma S. cc
    Advisors
    Merzaban, Jasmeen cc
    Committee members
    Li, Mo cc
    Mahfouz, Magdy M. cc
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2017-05
    Embargo End Date
    2018-05-07
    Permanent link to this record
    http://hdl.handle.net/10754/623399
    
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    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2018-05-07.
    Abstract
    Recruitment of circulating cells towards target sites is primarily dependent on E-selectin receptor/ligand adhesive interactions. Glycosyltransferase (GTs) are involved in the creation of E-selectin ligands. A sialofucosylated terminal tetrasaccharide like glycan structure known as sialyl Lewis x (sLex), is the most recognized ligand by selectins. This structure is found on the surface of cancer cells and leukocytes but is often absent on the surface of many adult stem cell populations. In order to synthesize sLex, GTs must be endogenously expressed and remain active within the cells. Generally, these stem cells express terminal sialylated lactosamine structures on their glycoproteins which require the addition of alpha-(1,3)-fucose to be converted into an E-selectin ligand. There are a number of fucosyltransferases (FUTs) that are able to modify terminal lactosamine structures to create sLex such as FUT6. In this work we focused on expressing and purifying active recombinant FUTs as a tool to help create sLex structures on the surface of adult stem cells in order to enhance their migration.
    Citation
    Al-Amoodi, A. S. (2017). Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X. KAUST Research Repository. https://doi.org/10.25781/KAUST-28D97
    DOI
    10.25781/KAUST-28D97
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-28D97
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Theses; Theses

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