Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X
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MS Thesis_Asma Alamoodi_Spring 2017.pdf
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MS Thesis_Asma
Type
ThesisAuthors
Al-Amoodi, Asma S.
Advisors
Merzaban, Jasmeen
Committee members
Li, Mo
Mahfouz, Magdy M.

Program
BioscienceDate
2017-05Embargo End Date
2018-05-07Permanent link to this record
http://hdl.handle.net/10754/623399
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At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2018-05-07.Abstract
Recruitment of circulating cells towards target sites is primarily dependent on E-selectin receptor/ligand adhesive interactions. Glycosyltransferase (GTs) are involved in the creation of E-selectin ligands. A sialofucosylated terminal tetrasaccharide like glycan structure known as sialyl Lewis x (sLex), is the most recognized ligand by selectins. This structure is found on the surface of cancer cells and leukocytes but is often absent on the surface of many adult stem cell populations. In order to synthesize sLex, GTs must be endogenously expressed and remain active within the cells. Generally, these stem cells express terminal sialylated lactosamine structures on their glycoproteins which require the addition of alpha-(1,3)-fucose to be converted into an E-selectin ligand. There are a number of fucosyltransferases (FUTs) that are able to modify terminal lactosamine structures to create sLex such as FUT6. In this work we focused on expressing and purifying active recombinant FUTs as a tool to help create sLex structures on the surface of adult stem cells in order to enhance their migration.Citation
Al-Amoodi, A. S. (2017). Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X. KAUST Research Repository. https://doi.org/10.25781/KAUST-28D97ae974a485f413a2113503eed53cd6c53
10.25781/KAUST-28D97