Show simple item record

dc.contributor.authorDiaz Rua, Ruben
dc.contributor.authorKeijer, Jaap
dc.contributor.authorPalou, Andreu
dc.contributor.authorvan Schothorst, Evert M.
dc.contributor.authorOliver, Paula
dc.date.accessioned2017-04-30T10:17:00Z
dc.date.available2017-04-30T10:17:00Z
dc.date.issued2017-04-19
dc.identifier.citationDíaz-Rúa R, Keijer J, Palou A, van Schothorst EM, Oliver P (2017) Long-term intake of a high-protein diet increases liver triacylglycerol deposition pathways and hepatic signs of injury in rats. The Journal of Nutritional Biochemistry 46: 39–48. Available: http://dx.doi.org/10.1016/j.jnutbio.2017.04.008.
dc.identifier.issn0955-2863
dc.identifier.doi10.1016/j.jnutbio.2017.04.008
dc.identifier.urihttp://hdl.handle.net/10754/623292
dc.description.abstractIntake of high-protein (HP) diets has increased over the last years, mainly due to their popularity for body weight control. Liver is the main organ handling ingested macronutrients and it is associated with the beginning of different pathologies. We aimed to deepen our knowledge on molecular pathways affected by long-term intake of an HP diet. We performed a transcriptome analysis on liver of rats chronically fed with a casein-rich HP diet and analyzed molecular parameters related to liver injury. Chronic increase in the dietary protein/carbohydrate ratio up-regulated processes related with amino acid uptake/metabolism and lipid synthesis, promoting a molecular environment indicative of hepatic triacylglycerol (TG) deposition. Moreover, changes in expression of genes involved in acid–base maintenance and oxidative stress indicate alterations in the pH balance due to the high acid load of the diet, which has been linked to liver/health damage. Up-regulation of immune-related genes was also observed. In concordance with changes at gene expression level, we observed increased liver TG content and increased serum markers of hepatic injury/inflammation (aspartate transaminase, C-reactive protein and TNF-alpha). Moreover, the HP diet strongly increased hepatic mRNA and protein levels of HSP90, a marker of liver injury. Thus, we show for the first time that long-term consumption of an HP diet, resulting in a high acid load, results in a hepatic transcriptome signature reflecting increased TG deposition and increased signs of health risk (increased inflammation, alterations in the acid–base equilibrium and oxidative stress). Persistence of this altered metabolic status could have unhealthy consequences.
dc.description.sponsorshipWe thank Enzo Ceresi and Paula Núñez for technical assistance in the immunohistochemistry and Western blot analysis, respectively.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0955286316303333
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in The Journal of Nutritional Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in The Journal of Nutritional Biochemistry, [, , (2017-04-19)] DOI: 10.1016/j.jnutbio.2017.04.008 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLiver steatosis
dc.subjectLiver disease
dc.subjectHyperproteic diet
dc.subjectAcid load
dc.subjectMicroarray analysis
dc.titleLong-term intake of a high protein diet increases liver triacylglycerol deposition pathways and hepatic signs of injury in rats
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalThe Journal of Nutritional Biochemistry
dc.eprint.versionPost-print
dc.contributor.institutionLaboratory of Molecular Biology, Nutrition and Biotechnology, Universitat de les Illes Balears and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain
dc.contributor.institutionHuman and Animal Physiology, Wageningen University, Wageningen, the Netherlands
kaust.personDiaz Rua, Ruben
refterms.dateFOA2018-04-19T00:00:00Z
dc.date.published-online2017-04-19
dc.date.published-print2017-08


Files in this item

Thumbnail
Name:
1-s2.0-S0955286316303333-main.pdf
Size:
1.452Mb
Format:
PDF
Description:
Accepted Manuscript

This item appears in the following Collection(s)

Show simple item record