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    Cardiac Progenitor Cell Extraction from Human Auricles

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    Type
    Book Chapter
    Authors
    Di Nardo, Paolo
    Pagliari, Francesca cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Physical Science and Engineering (PSE) Division
    Date
    2017-02-23
    Online Publication Date
    2017-02-23
    Print Publication Date
    2017
    Permanent link to this record
    http://hdl.handle.net/10754/623194
    
    Metadata
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    Abstract
    For many years, myocardial tissue has been considered terminally differentiated and, thus, incapable of regenerating. Recent studies have shown, instead, that cardiomyocytes, at least in part, are slowly substituted by new cells originating by precursor cells mostly embedded into the heart apex and in the atria. We have shown that an elective region of progenitor cell embedding is represented by the auricles, non-contractile atria appendages that can be easily sampled without harming the patient. The protocol here reported describes how from auricles a population of multipotent, cardiogenic cells can be isolated, cultured, and differentiated. Further studies are needed to fully exploit this cell population, but, sampling auricles, it could be possible to treat cardiac patients using their own cells circumventing rejection or organ shortage limitations.
    Citation
    Di Nardo P, Pagliari F (2017) Cardiac Progenitor Cell Extraction from Human Auricles. Methods in Molecular Biology: 145–154. Available: http://dx.doi.org/10.1007/978-1-4939-6756-8_11.
    Publisher
    Springer Nature
    Journal
    Methods in Molecular Biology
    DOI
    10.1007/978-1-4939-6756-8_11
    Additional Links
    http://link.springer.com/chapter/10.1007/978-1-4939-6756-8_11
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-4939-6756-8_11
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Physical Science and Engineering (PSE) Division; Book Chapters

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