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dc.contributor.authorAlshareef, Sahar
dc.contributor.authorLing, Yu
dc.contributor.authorButt, Haroon
dc.contributor.authorMariappan, Kiruthiga G.
dc.contributor.authorBenhamed, Moussa
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2017-04-10T07:49:50Z
dc.date.available2017-04-10T07:49:50Z
dc.date.issued2017-03-27
dc.identifier.citationAlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.
dc.identifier.issn1471-2164
dc.identifier.doi10.1186/s12864-017-3656-z
dc.identifier.urihttp://hdl.handle.net/10754/623088
dc.description.abstractBackground Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.
dc.description.sponsorshipWe wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Pedro Rodriguez for providing Arabidopsis seeds for several ABA mutants. We wish to thank Paula Duque for providing Arabidopsis seeds for several sr45-1 mutants. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAPKKK18
dc.publisherSpringer Nature
dc.relation.urlhttp://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3656-z
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlternative splicing
dc.subjectGEX1A
dc.subjectPladienolide B
dc.subjectSplicing inhibitors
dc.subjectSR proteins
dc.subjectAbiotic stress responses
dc.subjectABA
dc.titleHerboxidiene triggers splicing repression and abiotic stress responses in plants
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentLaboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology
dc.identifier.journalBMC Genomics
dc.eprint.versionPublisher's Version/PDF
kaust.personAlshareef, Sahar
kaust.personLing, Yu
kaust.personButt, Haroon
kaust.personMariappan, Kiruthiga G.
kaust.personBenhamed, Moussa
kaust.personMahfouz, Magdy M.
dc.relation.isSupplementedByAlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597
dc.relation.isSupplementedByDOI:10.6084/m9.figshare.c.3727597
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624149
refterms.dateFOA2018-06-14T00:06:24Z


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.