High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Abstract
Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.

Citation
Kremb S, Voolstra CR (2017) High-resolution phenotypic profiling of natural products-induced effects on the single-cell level. Scientific Reports 7: 44472. Available: http://dx.doi.org/10.1038/srep44472.

Acknowledgements
We thank the Bioscience core lab for supporting cell culture work and providing the Cellomics ArrayScan HCS instrument. Research in this publication was supported by baseline funds to CRV from King Abdullah University of Science and Technology (KAUST).

Publisher
Springer Nature

Journal
Scientific Reports

DOI
10.1038/srep44472

Additional Links
http://www.nature.com/articles/srep44472

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