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dc.contributor.authorAraujo, Paula Alexandra Da Silva
dc.contributor.authorMachado, Idalina
dc.contributor.authorMeireles, Ana
dc.contributor.authorLeiknes, TorOve
dc.contributor.authorMergulhão, Filipe
dc.contributor.authorMelo, Luís F.
dc.contributor.authorSimões, Manuel
dc.date.accessioned2017-03-05T06:13:15Z
dc.date.available2017-03-05T06:13:15Z
dc.date.issued2017-03-01
dc.identifier.citationAraújo PA, Machado I, Meireles A, Leiknes T, Mergulhão F, et al. (2017) Combination of selected enzymes with cetyltrimethylammonium bromide in biofilm inactivation, removal and regrowth. Food Research International. Available: http://dx.doi.org/10.1016/j.foodres.2017.02.016.
dc.identifier.issn0963-9969
dc.identifier.doi10.1016/j.foodres.2017.02.016
dc.identifier.urihttp://hdl.handle.net/10754/622956
dc.description.abstractEnzymes are considered an innovative and environmentally friendly approach for biofilm control due to their lytic and dispersal activities. In this study, four enzymes (β-glucanase, α-amylase, lipase and protease) were tested separately and in combination with the quaternary ammonium compound cetyltrimethylammonium bromide (CTAB) to control flow-generated biofilms of Pseudomonas fluorescens. The four enzymes caused modest reduction of biofilm colony forming units (CFU). Protease, β-glucanase and α-amylase also caused modest biofilm removal. CTAB combined with either β-glucanase or α-amylase increased biofilm removal. Its combination with either β-glucanase or protease increased CFU reduction. However, CTAB−protease combination was antagonist in biofilm removal. Long-term effects in biofilm mass reduction were observed after protease exposure. In contrast, biofilms treated with β-glucanase were able to regrowth significantly after exposure. Moreover, short-term respirometry tests with planktonic cells were performed to understand the effects of enzymes and their combination with CTAB on P. fluorescens viability. Protease and lipase demonstrated antimicrobial action, while α-amylase increased bacterial metabolic activity. The combination of CTAB with either protease or α-amylase was antagonistic, decreasing the antimicrobial action of CTAB. The overall results demonstrate a modest effect of the selected enzymes in biofilm control, either when applied alone or each one in combination with CTAB. Total biofilm removal or CFU reduction was not achieved and, in some cases, the use of enzymes antagonized the effects of CTAB. The results also propose that complementary tests, to characterize biofilm integrity and microbial viability, are required when someone is trying to assess the role of novel biocide - enzyme mixtures for effective biofilm control.
dc.description.sponsorshipThis work was the result of the project: i) POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy – UID/EQU/00511/2013) funded by the European Regional Development Fund (ERDF), through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by national funds, through FCT - Fundação para a Ciência e a Tecnologia. ii) NORTE-01-0145-FEDER-000005 – LEPABE-2-ECO-INNOVATION, supported by North Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). iii) PhD grant SFRH/BD/52624/2014. The authors also acknowledge King Abdullah University of Science and Technology (KAUST) for providing the financial support to Paula Araújo and Novozymes (Denmark) for providing the enzymes tested.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0963996917300790
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Food Research International. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Food Research International, [, , (2017-03-01)] DOI: 10.1016/j.foodres.2017.02.016 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntimicrobial quenching
dc.subjectBiofilm control
dc.subjectCleaning
dc.subjectDisinfection
dc.subjectEnzymes
dc.subjectPseudomonas fluorescens
dc.subjectFlow-generated biofilms
dc.titleCombination of selected enzymes with cetyltrimethylammonium bromide in biofilm inactivation, removal and regrowth
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalFood Research International
dc.eprint.versionPost-print
dc.contributor.institutionLEPABE, Department of Chemical Engineering, Faculty of Engineering (FEUP), University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal
kaust.personAraujo, Paula Alexandra Da Silva
kaust.personLeiknes, TorOve
refterms.dateFOA2018-03-01T00:00:00Z
dc.date.published-online2017-03-01
dc.date.published-print2017-05


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