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dc.contributor.authorSabermanesh, Kasra
dc.contributor.authorHoltham, Luke R.
dc.contributor.authorGeorge, Jessey
dc.contributor.authorRoessner, Ute
dc.contributor.authorBoughton, Berin A.
dc.contributor.authorHeuer, Sigrid
dc.contributor.authorTester, Mark A.
dc.contributor.authorPlett, Darren C.
dc.contributor.authorGarnett, Trevor P.
dc.date.accessioned2017-02-12T13:38:50Z
dc.date.available2017-02-12T13:38:50Z
dc.date.issued2017-04-07
dc.identifier.citationSabermanesh K, Holtham LR, George J, Roessner U, Boughton BA, et al. (2017) The transition from a maternal to external nitrogen source in maize seedlings. Journal of Integrative Plant Biology. Available: http://dx.doi.org/10.1111/jipb.12525.
dc.identifier.issn1672-9072
dc.identifier.doi10.1111/jipb.12525
dc.identifier.urihttp://hdl.handle.net/10754/622875
dc.description.abstractMaximising NO3 - uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 - uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 - uptake and metabolism, to gain an understanding of how the NO3 - uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed-derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilising eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilise until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3 - uptake capacity increases until shoot N% stabilises. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 - transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3 - uptake capacity to meet N demand provide an insight into the processes controlling N uptake.
dc.description.sponsorshipWe thank Hanne Thompson, Yuan Li, Nenah Mackenzie, Priyanka Reddy and Chia Ng for the technical assistance provided for this study. We also thank Metabolomics Australia, School of BioSciences, The University of Melbourne, for sample preparation and amino acid analysis. Ute Roessner and Berin Boughton are also grateful to Victorian Node of Metabolomics Australia, which is funded through Bioplatforms Australia Pty Ltd, a National Collaborative Research Infrastructure Strategy, 5.1 Biomolecular Platforms and informatics investment, and co-investment from the Victorian State Government and The University of Melbourne. This project was supported by the Australian Centre for Plant Functional Genomics, the Australian Research Council (LP130101055), DuPont Pioneer and the Grains Research and Development Corporation (GRS10437).
dc.publisherWiley
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jipb.12525/abstract
dc.rightsThis is the peer reviewed version of the following article: The transition from a maternal to external nitrogen source in maize seedlings, which has been published in final form at http://doi.org/10.1111/jipb.12525. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
dc.subjectZea mays
dc.subjectAmino acids
dc.subjectSeed
dc.subjectSeedling
dc.subjectNitrogen use efficiency
dc.subjectRoots
dc.subjectNitrate Uptake
dc.subjectNrt
dc.subjectNpf
dc.titleThe transition from a maternal to external nitrogen source in maize seedlings
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.identifier.journalJournal of Integrative Plant Biology
dc.eprint.versionPost-print
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute, University of Adelaide; Adelaide, SA 5064, Australia
dc.contributor.institutionSchool of Agriculture, Food and Wine; Waite Research Institute, University of Adelaide; Adelaide, SA 5064 Australia
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; School of BioSciences, University of Melbourne; Parkville, Vic. 3010 Australia
dc.contributor.institutionMetabolomics Australia; School of BioSciences, University of Melbourne; Vic. 3010 Australia
kaust.personTester, Mark A.
refterms.dateFOA2018-02-07T00:00:00Z
dc.date.published-online2017-04-07
dc.date.published-print2017-04


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