Show simple item record

dc.contributor.authorPerez, Jose E.
dc.contributor.authorRavasi, Timothy
dc.contributor.authorKosel, Jürgen
dc.date.accessioned2017-01-29T13:51:39Z
dc.date.available2017-01-29T13:51:39Z
dc.date.issued2016-12-28
dc.identifier.citationPerez JE, Ravasi T, Kosel J (2016) Mesenchymal stem cells cultured on magnetic nanowire substrates. Nanotechnology 28: 055703. Available: http://dx.doi.org/10.1088/1361-6528/aa52a3.
dc.identifier.issn0957-4484
dc.identifier.issn1361-6528
dc.identifier.doi10.1088/1361-6528/aa52a3
dc.identifier.urihttp://hdl.handle.net/10754/622787
dc.description.abstractStem cells have been shown to respond to extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array of iron nanowires (NWs) aligned perpendicularly to the surface was fabricated by pulsed electrodepositon in porous alumina templates followed by a partial removal of the alumina to reveal 2-3 μm of the NWs. This resulted in alumina substrates with densely arranged NWs of 33 nm in diameter separated by 100 nm. The substrates were characterized by scanning electron microscopy (SEM) energy dispersive x-ray analysis and vibrating sample magnetometer. The NW array was then used as a platform for the culture of human mesenchymal stem cells (hMSCs). The cells were stained for the cell nucleus and actin filaments, as well as immuno-stained for the focal adhesion protein vinculin, and then observed by fluorescence microscopy in order to characterize their spreading behavior. Calcein AM/ethidium homodimer-1 staining allowed the determination of cell viability. The interface between the cells and the NWs was studied using SEM. Results showed that hMSCs underwent a re-organization of actin filaments that translated into a change from an elongated to a spherical cell shape. Actin filaments and vinculin accumulated in bundles, suggesting the attachment and formation of focal adhesion points of the cells on the NWs. Though the overall number of cells attached on the NWs was lower compared to the control, the attached cells maintained a high viability (>90%) for up to 6 d. Analysis of the interface between the NWs and the cells confirmed the re-organization of F-actin and revealed the adhesion points of the cells on the NWs. Additionally, a net of filopodia surrounded each cell, suggesting the probing of the array to find additional adhesion points. The cells maintained their round shape for up to 6 d of culture. Overall, the NW array is a promising nanostructured platform for studying and influencing hMSCs differentiation.
dc.description.sponsorshipResearch reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).
dc.publisherIOP Publishing
dc.relation.urlhttp://iopscience.iop.org/article/10.1088/1361-6528/aa52a3/meta;jsessionid=227AD3FA331E525E13535E508C947DF4.c2.iopscience.cld.iop.org
dc.subjectbiocompatibility
dc.subjectcell culture
dc.subjectmagnetic nanowires
dc.subjectmesenchymal stem cells
dc.titleMesenchymal stem cells cultured on magnetic nanowire substrates
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalNanotechnology
kaust.personPerez, Jose E.
kaust.personRavasi, Timothy
kaust.personKosel, Jürgen


This item appears in the following Collection(s)

Show simple item record