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dc.contributor.authorRodio, Marina
dc.contributor.authorColuccino, Luca
dc.contributor.authorRomeo, Elisa
dc.contributor.authorGenovese, Alessandro
dc.contributor.authorDiaspro, Alberto
dc.contributor.authorGarau, Gianpiero
dc.contributor.authorIntartaglia, Romuald
dc.date.accessioned2017-01-29T13:51:37Z
dc.date.available2017-01-29T13:51:37Z
dc.date.issued2017
dc.identifier.citationRodio M, Coluccino L, Romeo E, Genovese A, Diaspro A, et al. (2017) Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity. J Mater Chem B 5: 279–288. Available: http://dx.doi.org/10.1039/C6TB02023B.
dc.identifier.issn2050-750X
dc.identifier.issn2050-7518
dc.identifier.doi10.1039/C6TB02023B
dc.identifier.urihttp://hdl.handle.net/10754/622764
dc.description.abstractHydroxyapatite bioactive complexes are being increasingly recognized as effective available means in regenerative medicine. Conventional technologies for their synthesis have drawbacks from a synthetic standpoint, mainly requiring high temperatures and multi-step processes. Here, we show that ultra-small hydroxyapatite conjugated-nanoparticles (Ha-CNPs) can be obtained at room temperature by Pulsed Laser Ablation (PLA) directly in protein solution using picosecond pulses at near infrared wavelengths. The results showed that the nanoparticle size was driven by the concentration of the protein. Using this approach, we obtained aqueous soluble and ultra-small crystalline nanoparticles of ≈3 nm diameter coated with protein molecules (surface coverage ≈ 5.5 pmol cm; zeta potential ≈-33.5 mV). These nanoparticles showed low cytotoxicity in vitro compared to chemically synthesized nanoparticles, and revealed proliferative and osteoinductive effects on human bone marrow mesenchymal stem cells (hMSCs). The resulting enhanced cell osteogenic differentiation suggested that our PLA-based synthetic approach might be exploited in novel applications of regenerative medicine.
dc.description.sponsorshipThis work was supported by Istituto Italiano di Tecnologia. The authors gratefully acknowledge S. Marras and A. Scarpellini for X-ray diffraction and energy-dispersive X-ray spectroscopy measurements, respectively.
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2017/TB/C6TB02023B
dc.titleFacile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalJ. Mater. Chem. B
dc.contributor.institutionNanophysics, Istituto Italiano di Tecnologia, via Morego 30, Genova, 16163, Italy
dc.contributor.institutionD3 validation, Drug Discovery and Development, Istituto Italiano di Tecnologia, via Morego 30, Genova, 16163, Italy
dc.contributor.institutionNanochemistry, Istituto Italiano di Tecnologia, via Morego 30, Genova, 16163, Italy
dc.contributor.institutionCenter for Nanotechnology Innovation, Istituto Italiano di Tecnologia, Piazza San Silvetro 12, Pisa, 56124, Italy
kaust.personGenovese, Alessandro


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