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    Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity

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    Type
    Article
    Authors
    Rodio, Marina
    Coluccino, Luca
    Romeo, Elisa
    Genovese, Alessandro
    Diaspro, Alberto
    Garau, Gianpiero
    Intartaglia, Romuald
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2016-11-24
    Permanent link to this record
    http://hdl.handle.net/10754/622764
    
    Metadata
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    Abstract
    Hydroxyapatite bioactive complexes are being increasingly recognized as effective available means in regenerative medicine. Conventional technologies for their synthesis have drawbacks from a synthetic standpoint, mainly requiring high temperatures and multi-step processes. Here, we show that ultra-small hydroxyapatite conjugated-nanoparticles (Ha-CNPs) can be obtained at room temperature by Pulsed Laser Ablation (PLA) directly in protein solution using picosecond pulses at near infrared wavelengths. The results showed that the nanoparticle size was driven by the concentration of the protein. Using this approach, we obtained aqueous soluble and ultra-small crystalline nanoparticles of ≈3 nm diameter coated with protein molecules (surface coverage ≈ 5.5 pmol cm; zeta potential ≈-33.5 mV). These nanoparticles showed low cytotoxicity in vitro compared to chemically synthesized nanoparticles, and revealed proliferative and osteoinductive effects on human bone marrow mesenchymal stem cells (hMSCs). The resulting enhanced cell osteogenic differentiation suggested that our PLA-based synthetic approach might be exploited in novel applications of regenerative medicine.
    Citation
    Rodio M, Coluccino L, Romeo E, Genovese A, Diaspro A, et al. (2017) Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity. J Mater Chem B 5: 279–288. Available: http://dx.doi.org/10.1039/C6TB02023B.
    Sponsors
    This work was supported by Istituto Italiano di Tecnologia. The authors gratefully acknowledge S. Marras and A. Scarpellini for X-ray diffraction and energy-dispersive X-ray spectroscopy measurements, respectively.
    Publisher
    Royal Society of Chemistry (RSC)
    Journal
    J. Mater. Chem. B
    ISSN
    2050-750X
    2050-7518
    DOI
    10.1039/C6TB02023B
    Additional Links
    http://pubs.rsc.org/en/Content/ArticleLanding/2017/TB/C6TB02023B
    ae974a485f413a2113503eed53cd6c53
    10.1039/C6TB02023B
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division

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