Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity
Type
ArticleAuthors
Rodio, MarinaColuccino, Luca
Romeo, Elisa
Genovese, Alessandro
Diaspro, Alberto
Garau, Gianpiero
Intartaglia, Romuald
Date
2017Permanent link to this record
http://hdl.handle.net/10754/622764
Metadata
Show full item recordAbstract
Hydroxyapatite bioactive complexes are being increasingly recognized as effective available means in regenerative medicine. Conventional technologies for their synthesis have drawbacks from a synthetic standpoint, mainly requiring high temperatures and multi-step processes. Here, we show that ultra-small hydroxyapatite conjugated-nanoparticles (Ha-CNPs) can be obtained at room temperature by Pulsed Laser Ablation (PLA) directly in protein solution using picosecond pulses at near infrared wavelengths. The results showed that the nanoparticle size was driven by the concentration of the protein. Using this approach, we obtained aqueous soluble and ultra-small crystalline nanoparticles of ≈3 nm diameter coated with protein molecules (surface coverage ≈ 5.5 pmol cm; zeta potential ≈-33.5 mV). These nanoparticles showed low cytotoxicity in vitro compared to chemically synthesized nanoparticles, and revealed proliferative and osteoinductive effects on human bone marrow mesenchymal stem cells (hMSCs). The resulting enhanced cell osteogenic differentiation suggested that our PLA-based synthetic approach might be exploited in novel applications of regenerative medicine.Citation
Rodio M, Coluccino L, Romeo E, Genovese A, Diaspro A, et al. (2017) Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity. J Mater Chem B 5: 279–288. Available: http://dx.doi.org/10.1039/C6TB02023B.Sponsors
This work was supported by Istituto Italiano di Tecnologia. The authors gratefully acknowledge S. Marras and A. Scarpellini for X-ray diffraction and energy-dispersive X-ray spectroscopy measurements, respectively.Publisher
Royal Society of Chemistry (RSC)Journal
Journal of Materials Chemistry BAdditional Links
http://pubs.rsc.org/en/Content/ArticleLanding/2017/TB/C6TB02023Bae974a485f413a2113503eed53cd6c53
10.1039/C6TB02023B