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dc.contributor.authorCifre, Margalida
dc.contributor.authorDiaz Rua, Ruben
dc.contributor.authorVarela-Calviño, Rubén
dc.contributor.authorReynés, Bàrbara
dc.contributor.authorPericás-Beltrán, Jordi
dc.contributor.authorPalou, Andreu
dc.contributor.authorOliver, Paula
dc.date.accessioned2017-01-29T13:51:37Z
dc.date.available2017-01-29T13:51:37Z
dc.date.issued2016-12-30
dc.identifier.citationCifre M, Díaz-Rúa R, Varela-Calviño R, Reynés B, Pericás-Beltrán J, et al. (2016) Human peripheral blood mononuclear cell in vitro system to test the efficacy of food bioactive compounds: Effects of polyunsaturated fatty acids and their relation with BMI. Molecular Nutrition & Food Research: 1600353. Available: http://dx.doi.org/10.1002/mnfr.201600353.
dc.identifier.issn1613-4125
dc.identifier.doi10.1002/mnfr.201600353
dc.identifier.urihttp://hdl.handle.net/10754/622761
dc.description.abstractScope: To analyse the usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n-3 long-chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are body mass index (BMI) dependent. Methods and results: PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination. PBMC reflected increased beta-oxidation-like capacity (CPT1A expression) in OW/OB but only after DHA treatment. However, insensitivity to n-3 LCPUFA was evident in OW/OB for lipogenic genes: both PUFA diminished FASN and SREBP1C expression in NW, but no effect was observed for DHA in PBMC from high-BMI subjects. This insensitivity was also evident for inflammation gene profile: all treatments inhibited key inflammatory genes in NW; nevertheless, no effect was observed in OW/OB after DHA treatment, and EPA effect was impaired. SLC27A2, IL6 and TNFα PBMC expression analysis resulted especially interesting to determine obesity-related n-3 LCPUFA insensitivity. Conclusion: A PBMC-based human in vitro system reflects n-3 LCPUFA effects on lipid metabolism/inflammation which is impaired in OW/OB. These results confirm the utility of PBMC ex vivo systems for bioactive-compound screening to promote functional food development and to establish appropriate dietary strategies for obese population.
dc.description.sponsorshipA.P and P.O conceived the experiment. M.C did the major part of the experimental work. R.D-R, R.V-C, B.R and J.P-B were involved in the experimental set-up, experimental work and sample collection. M.C and P.O wrote the article; all the authors participated in critical revision of the manuscript. We thank Enzo Ceresi for technical assistance in the determination of PBMC cell populations. CIBER de Fisiopatología de la Obesidad y Nutrición is an initiative of the ISCIII. This work was supported by the Spanish Government (Ministerio de Economía y Competititividad, INTERBIOBES -AGL2015-67019-P- and EPIMILK -AGL2012-33692-) and by the University of the Balearic Islands (IBIFLEX, FA38/2016). The Laboratory of Molecular Biology, Nutrition and Biotechnology is member of the European Research Network of Excellence NuGO (The European Nutrigenomics Organization, EU Contract: FOOD-CT-2004-506360 NUGO). MC is a recipient of a fellowship from the Spanish Government.
dc.publisherWiley
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/mnfr.201600353/full
dc.subjectBiomarkers
dc.subjectFood bioactive compounds
dc.subjectObesity
dc.subjectPeripheral blood mononuclear cells
dc.subjectPolyunsaturated fatty acids
dc.titleHuman peripheral blood mononuclear cell in vitro system to test the efficacy of food bioactive compounds: Effects of polyunsaturated fatty acids and their relation with BMI
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalMolecular Nutrition & Food Research
dc.contributor.institutionLaboratory of Molecular Biology, Nutrition and Biotechnology Universitat de les Illes Balears and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn) Palma de Mallorca Spain
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology University of Santiago de Compostela Santiago de Compostela Spain
dc.contributor.institutionResearch Group on Evidence, Lifestyles and Health Universitat de les Illes Balears Palma de Mallorca Spain
kaust.personDiaz Rua, Ruben
dc.date.published-online2016-12-30
dc.date.published-print2017-04


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