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dc.contributor.authorLing, Yu
dc.contributor.authorAlshareef, Sahar
dc.contributor.authorButt, Haroon
dc.contributor.authorLozano-Juste, Jorge
dc.contributor.authorLi, Lixin
dc.contributor.authorGalal, Aya A.
dc.contributor.authorMoustafa, Ahmed
dc.contributor.authorMomin, Afaque Ahmad Imtiyaz
dc.contributor.authorTashkandi, Manal
dc.contributor.authorRichardson, Dale N.
dc.contributor.authorFujii, Hiroaki
dc.contributor.authorArold, Stefan T.
dc.contributor.authorRodriguez, Pedro L.
dc.contributor.authorDuque, Paula
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2017-01-29T13:51:36Z
dc.date.available2017-01-29T13:51:36Z
dc.date.issued2016-09-24
dc.identifier.citationLing Y, Alshareef S, Butt H, Lozano-Juste J, Li L, et al. (2017) Pre-mRNA splicing repression triggers abiotic stress signaling in plants. The Plant Journal. Available: http://dx.doi.org/10.1111/tpj.13383.
dc.identifier.issn0960-7412
dc.identifier.doi10.1111/tpj.13383
dc.identifier.urihttp://hdl.handle.net/10754/622750
dc.description.abstractAlternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A
dc.description.sponsorshipWe wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.
dc.publisherWiley-Blackwell
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13383/full
dc.subjectAbscisic acid
dc.subjectAlternative splicing
dc.subjectAbiotic Stress Responses
dc.subjectSerine/arginine-rich Proteins
dc.subjectPladienolide B
dc.subjectSplicing Inhibitors
dc.titlePre-mRNA splicing repression triggers abiotic stress signaling in plants
dc.typeArticle
dc.contributor.departmentLaboratory for Genome Engineering; Division of Biological Sciences; 4700 King Abdullah University of Science and Technology; Thuwal 23955-6900 Saudi Arabia
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalThe Plant Journal
dc.contributor.institutionInstituto de Biologia Molecular y Celular de Plantas; Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia; ES-46022 Valencia Spain
dc.contributor.institutionDepartment of Biology and Biotechnology Graduate Program; American University in Cairo; New Cairo 11835 Egypt
dc.contributor.institutionInstituto Gulbenkian de Ciência; Rua da Quinta Grande 6 2780-156 Oeiras Portugal
dc.contributor.institutionMolecular Plant Biology; Department of Biochemistry; University of Turku; FI-20014 Turku Finland
kaust.personLing, Yu
kaust.personAlshareef, Sahar
kaust.personButt, Haroon
kaust.personLi, Lixin
kaust.personMomin, Afaque Ahmad Imtiyaz
kaust.personTashkandi, Manal
kaust.personArold, Stefan T.
kaust.personMahfouz, Magdy M.


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