Pre-mRNA splicing repression triggers abiotic stress signaling in plants.
Galal, Aya A.
Momin, Afaque Ahmad Imtiyaz
Richardson, Dale N.
Arold, Stefan T.
Rodriguez, Pedro L.
Mahfouz, Magdy M.
KAUST DepartmentAcademic Affairs
Biological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
Lab Consumables & Gases
Laboratory for Genome Engineering
Office of the VP
Structural Biology and Engineering
Online Publication Date2017-01-17
Print Publication Date2017-01
Embargo End Date2018-01-17
Permanent link to this recordhttp://hdl.handle.net/10754/622750
MetadataShow full item record
AbstractAlternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A
CitationLing, Y., Alshareef, S., Butt, H., Lozano-Juste, J., Li, L., Galal, A. A., … Mahfouz, M. M. (2017). Pre-mRNA splicing repression triggers abiotic stress signaling in plants. The Plant Journal, 89(2), 291–309. doi:10.1111/tpj.13383
SponsorsWe wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.
JournalThe Plant Journal