StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity
AuthorsSamodelov, S. L.
Beyer, H. M.
Ebenho h, O.
Zurbriggen, M. D.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Plant Science Program
Online Publication Date2016-11-04
Print Publication Date2016-11-04
Permanent link to this recordhttp://hdl.handle.net/10754/622741
MetadataShow full item record
AbstractStrigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.
CitationSamodelov SL, Beyer HM, Guo X, Augustin M, Jia K-P, et al. (2016) StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity. Science Advances 2: e1601266–e1601266. Available: http://dx.doi.org/10.1126/sciadv.1601266.
SponsorsThis research was supported by funding from the excellence initiatives of the German federal and state governments (DFG EXC-1028-CEPLAS, EXC 294-BIOSS, and GSC 4-SGBM) and the King Abdullah University of Science and Technology (KAUST).
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Showing items related by title, author, creator and subject.
Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stressLiu, Junwei; He, Hanzi; Vitali, Marco; Visentin, Ivan; Charnikhova, Tatsiana V.; Haider, Imran; Schubert, Andrea; Ruyter-Spira, Carolien P.; Bouwmeester, Harro J J; Lovisolo, Claudio; Cardinale, Francesca (Planta, Springer Nature, 2015-02-26) [Article]Main conclusion: Strigolactone changes and cross talk with ABA unveil a picture of root-specific hormonal dynamics under stress.Abstract: Strigolactones (SLs) are carotenoid-derived hormones influencing diverse aspects of development and communication with (micro)organisms, and proposed as mediators of environmental stimuli in resource allocation processes; to contribute to adaptive adjustments, therefore, their pathway must be responsive to environmental cues. To investigate the relationship between SLs and abiotic stress in Lotus japonicus, we compared wild-type and SL-depleted plants, and studied SL metabolism in roots stressed osmotically and/or phosphate starved. SL-depleted plants showed increased stomatal conductance, both under normal and stress conditions, and impaired resistance to drought associated with slower stomatal closure in response to abscisic acid (ABA). This confirms that SLs contribute to drought resistance in species other than Arabidopsis. However, we also observed that osmotic stress rapidly and strongly decreased SL concentration in tissues and exudates of wild-type Lotus roots, by acting on the transcription of biosynthetic and transporter-encoding genes and independently of phosphate abundance. Pre-treatment with exogenous SLs inhibited the osmotic stress-induced ABA increase in wild-type roots and down-regulated the transcription of the ABA biosynthetic gene LjNCED2. We propose that a transcriptionally regulated, early SL decrease under osmotic stress is needed (but not sufficient) to allow the physiological increase of ABA in roots. This work shows that SL metabolism and effects on ABA are seemingly opposite in roots and shoots under stress.
Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesisZhang, Yanxia; van Dijk, Aalt D J; Scaffidi, Adrian; Flematti, Gavin R.; Hofmann, Manuel; Charnikhova, Tatsiana; Verstappen, Francel; Hepworth, Jo; van der Krol, Sander; Leyser, Ottoline; Smith, Steven M.; Zwanenburg, Binne; Al-Babili, Salim; Ruyter-Spira, Carolien; Bouwmeester, Harro J. (Nature Chemical Biology, Springer Nature, 2014-10-26) [Article]Strigolactones (SLs) are a class of phytohormones and rhizosphere signaling compounds with high structural diversity. Three enzymes, carotenoid isomerase DWARF27 and carotenoid cleavage dioxygenases CCD7 and CCD8, were previously shown to convert all-trans-β-carotene to carlactone (CL), the SL precursor. However, how CL is metabolized to SLs has remained elusive. Here, by reconstituting the SL biosynthetic pathway in Nicotiana benthamiana, we show that a rice homolog of Arabidopsis More Axillary Growth 1 (MAX1), encodes a cytochrome P450 CYP711 subfamily member that acts as a CL oxidase to stereoselectively convert CL into ent-2'-epi-5-deoxystrigol (B-C lactone ring formation), the presumed precursor of rice SLs. A protein encoded by a second rice MAX1 homolog then catalyzes the conversion of ent-2'-epi-5-deoxystrigol to orobanchol. We therefore report that two members of CYP711 enzymes can catalyze two distinct steps in SL biosynthesis, identifying the first enzymes involved in B-C ring closure and a subsequent structural diversification step of SLs.
Additional file 1: of Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesisButt, Haroon; Jamil, Muhammad; Wang, Jian You; Al-Babili, Salim; Mahfouz, Magdy M. (figshare, 2018) [Dataset]Supplementary Information. (DOCX 65982 kb)