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dc.contributor.authorFerraro, Daniela
dc.contributor.authorTredici, Ilenia G.
dc.contributor.authorGhigna, Paolo
dc.contributor.authorCastillio-Michel, Hiram
dc.contributor.authorFalqui, Andrea
dc.contributor.authorDi Benedetto, Cristiano
dc.contributor.authorAlberti, Giancarla
dc.contributor.authorRicci, Vittorio
dc.contributor.authorAnselmi-Tamburini, Umberto
dc.contributor.authorSommi, Patrizia
dc.date.accessioned2017-01-25T08:57:40Z
dc.date.available2017-01-25T08:57:40Z
dc.date.issued2017
dc.identifier.citationFerraro D, Tredici IG, Ghigna P, Castillio-Michel H, Falqui A, et al. (2017) Dependence of the Ce(iii)/Ce(iv) ratio on intracellular localization in ceria nanoparticles internalized by human cells. Nanoscale. Available: http://dx.doi.org/10.1039/c6nr07701c.
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.doi10.1039/c6nr07701c
dc.identifier.urihttp://hdl.handle.net/10754/622724
dc.description.abstractCeO2 nanoparticles (CNPs) have been investigated as promising antioxidant agents with significant activity in the therapy of diseases involving free radicals or oxidative stress. However, the exact mechanism responsible for CNP activity has not been completely elucidated. In particular, in situ evidence of modification of the oxidative state of CNPs in human cells and their evolution during cell internalization and subsequent intracellular distribution has never been presented. In this study we investigated modification of the Ce(iii)/Ce(iv) ratio following internalization in human cells by X-ray absorption near edge spectroscopy (XANES). From this analysis on cell pellets, we observed that CNPs incubated for 24 h showed a significant increase in Ce(iii). By coupling on individual cells synchrotron micro-X-ray fluorescence (μXRF) with micro-XANES (μXANES) we demonstrated that the Ce(iii)/Ce(iv) ratio is also dependent on CNP intracellular localization. The regions with the highest CNP concentrations, suggested to be endolysosomes by transmission electron microscopy, were characterized by Ce atoms in the Ce(iv) oxidation state, while a higher Ce(iii) content was observed in regions surrounding these areas. These observations suggest that the interaction of CNPs with cells involves a complex mechanism in which different cellular areas play different roles.
dc.description.sponsorshipWe thank A. Casu (King Adullah University of Science and Technology, Thuwal) for the single-crystal structure analysis and E. Solcia (University of Pavia, Department of Molecular Medicine) for the ultrastructural cell analysis. This work was supported by Fondazione Cariplo (Grants No. 2011-2095).
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C6NR07701C#!divAbstract
dc.rightsThis article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleDependence of the Ce(iii)/Ce(iv) ratio on intracellular localization in ceria nanoparticles internalized by human cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalNanoscale
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, Italy. patrizia.sommi@unipv.it.
dc.contributor.institutionDepartment of Chemistry, University of Pavia, 27100 Pavia, Italy.
dc.contributor.institutionEuropean Synchrotron Radiation Facility, 38000 Grenoble, France.
kaust.personFalqui, Andrea
kaust.personDi Benedetto, Cristiano
refterms.dateFOA2018-06-13T14:54:14Z


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